Solid phase purification and SSCP analysis of amplified genomic DNA by capillary electrophoresis

Silvana Debernardi, Massimo Luzzana and Gianluca De Bellis

Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, L.I.T.A., Via Fratelli Cervi 93, 20090 Segrate, ITALY
Email debellis@sun2.itba.mi.cnr.it
[Received 11/28/95; Accepted 12/25/95; On-line 1/1/96]

Abstract

Detection and identification of point mutations in genomic DNA has proven increasingly important in biomedical research. A variety of methods for the analysis of single base substitutions have been proposed among which Single Strand Conformational Polymorphism (SSCP) quickly gained success due to its simplicity.

In this work we present an analytical on-line tool which combines the ease of solid phase purification of amplified genomic DNA, the simplicity of SSCP and the significant potential advantages offered by capillary electrophoresis (CE).