[Frontiers in Bioscience 1, c4-15, November 1, 1996]
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CAVEAT LECTOR



THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

Gerard J. Nuovo

Director, MGN Medical Research Laboratories, Setauket, New York 11733, USA

Received: 07/25/96; Accepted: 10/02/96; On-line 11/01/96

7. REFERENCES

1. GJ Nuovo: PCR in situ hybridization: Protocols and Applications, third edition. Lippincott, Raven Press, New York. 538, (1996)

2. AA Long, P Komminoth, E Lee, & HJ Wolfe: Comparison of indirect and direct in situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations. Histochemistry 99, 151-62, (1993)

3. J Sallstrom, M Alemi & E Wilander: Pitfalls of in situ PCR using direct incorporation of labeled nucleotides. Anticancer Res 13, 1153-5, (1993)

4. O Bagasra, T Sheshamma & R Pomeranz: Polymerase chain reaction in situ: Intracellular amplification and detection of HIV-1 proviral DNA and other specific genes. J Immunol Methods 158, 131-45, (1993)

5. RH Chen & SV Fuggle: in situ cDNA polymerase chain reaction. A novel technique for detecting mRNA expression. Am J Pathol 143, 1527-34, (1993)

6. J Gosden & D Hanratty: PCR in situ: A rapid alternative to in situ hybridization for mapping short, low copy number sequences without isotopes. Biotechniques 15, 78-80, (1993)

7. BW Heniford: Variation in cellular EGF receptor mRNA expression demonstrated by in situ RT polymerase chain reaction. Nucl Acid Res 31, 3159-66, (1993)

8. A Martinez & F Cuttitta: Non radioactive localization of nucleic acids by in situ PCR and in situ RT PCR in paraffin embedded sections. J Histochem Cytochem 43, 739-47, (1995)

9. A Metha: Detection of herpes simplex virus sequences in the trigeminal ganglia of latently infected mice by and in situ PCR method. Cell Vision 1, 110-15, (1994)

10. JJ O'leary: Importance of fixation procedures on DNA template and its suitability for solution phase PCR and PCR in situ hybridization. Histochem J 26, 337-46, (1994)

11. V Patel, B Heniford, T Weiman & F Hendler: Detection of epidermal growth factor receptor mRNA in tissue sections from biopsy specimens using in situ PCR. Am J Pathol 144, 7-14, (1994)

12. C Pestaner , M Bibbo, T Seshamma & O Bagasra: Potential of the in situ PCR reaction in diagnostic cytology. Acta Cytol 38, 676-80, (1994)

13. IA Teo & S Shaunak: PCR in situ: An appraisal of an emerging technique. Histochem J 27, 647-59, (1995)

14. K Ueki, EP Richardson & DN Louis: in situ PCR demonstration of JC virus in progressive multifocal leukoencephalopathy, including an index case. Annals Neurol 36, 670-3, (1994)

15. J Cheng & GJ Nuovo: The utility of RT in situ PCR in the diagnosis of viral infections. J Histotech 17, 247-51, (1994)

16. A Haase, EF Retzel & KA Staskus: Amplification and detection of lentiviral DNA inside cells. Proc Natl Acad Sci, USA 87, 4981-4, (1990)

17. GJ Nuovo & A Forde: An improved system for reverse transcriptase in situ PCR. J Histotech 18, 295-9, (1995)

18. GJ Nuovo & SJ Silverstein: Comparison of formalin, buffered formalin, and Bouin's fixation on the detection of human papillomavirus DNA extracted from genital lesions. Lab Invest 59, 720-4, (1988)

19. TR Moench, HE Gendelman, JE Clements, O Narayan & DE Griffin: Efficiency of in situ hybridization as a function of probe size and fixation technique. J Virol Methods 11, 119-30, (1985)

20. GJ Nuovo, F Gallery, R Hom, P MacConnell & W Bloch: Importance of different variables for optimizing in situ detection of PCR-amplified DNA. PCR Method Applic 2, 305-12, (1993)

21. GJ Nuovo, F Gallery & P MacConnell: Analysis of non-specific DNA synthesis during in situ PCR. PCR Meth Applic 4, 342-349, (1994)

22. CE Greer, SL Peterson & N.B.Manos: PCR amplification from paraffin-embedded tissues: Effects of fixative and fixative times. Am J Clin Pathol 95, 117-124, (1991)

23. GJ Nuovo, G Gorgone, P MacConnell & P Goravic: in situ localization of human and viral cDNAs after PCR-amplification. PCR Method Applic 2,117-3, (1992)

24. LA Liotta, PS Steeg & WG Stetler-Stevenson: Cancer metastasis and angiogenesis: An imbalance of positive and negative regulation. Cell 64, 327-36, (1991)

25. R Khokha, P Waterhouse, S Yagel, PK Lala, CM Overall, G Norton & DT Denhardt: Antisense RNA-induced reduction in murine TIMP levels confers oncogenicity on Swiss 3T3 cells. Science 243, 947-50, (1989)

26. GJ Nuovo, P MacConnell & DL French: Correlation of the in situ detection of PCR-amplified metalloprotease cDNAs and their inhibitors with prognosis in cervical carcinoma. Cancer Research 55, 267-75, (1995)

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