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SPERMINE STIMULATES THE PHOSPHORYLATION OF THE NUCLEAR MATRIX PROTEINS CATALYZED BY NUCLEAR KINASE II
Rati Verma and Kuang Yu Chen
Department of Chemistry and The Cancer Institute of New Jersey, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08855-0939
Received 8/19/97 Accepted 8/25/97
TABLE OF CONTENTS
- 1. Abstract
- 2. Introduction
- 3.Materials and Methods
- 3.1 Purification of NII nuclear kinase
- 3.2 Phosphorylation of nuclear fractions using exogenously added NII nuclear kinase
- 3.3 Other procedures
- 4. Results
- 4.1 Purification and characterization of NII nuclear kinase
- 4.2 NII kinase catalyzed phosphorylation of nuclear proteins
- 4.3 Phosphorylation of subnuclear fractions by NII nuclear kinase
- 5. Discussion
- 6. Acknowledgments
- 7. References
- 8. Entire manuscript
Key words: NII nuclear kinase, Spermine, Nuclear matrix, Phosphorylation
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