[Frontiers in Bioscience 1, c4-15, November 1, 1996]
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EXPERIMENTAL PROCEDURE FOR THE DETECTION OF A RARE HUMAN mRNA WITH THE DIG SYSTEM

Barbara Rueger, Julia Thalhammer, Irmgard Obermaier, and Stefanie Gruenewald-Janho

Boehringer Mannheim Customer Service Lab, International Support, Mannheim, Germany

TABLE OF CONTENTS

1. Abstract
2. Materials and methods
Electrophoresis conditions and sample preparation
RNA transfer
Probe labeling
Prehybridization, hybridization, and stringency washes
Chemiluminescent detection
3. Results and discussion
4. References
5. Entire manuscript