[Frontiers in Bioscience 2, c6-8, February 15, 1997]
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RAPID PURIFICATION OF HISTIDINE-TAGGED GLUTATHIONE S-TRANSFERASE FUSION PROTEIN BY METAL CHELATE POROS® PERFUSION CHROMATOGRAPHY® MEDIA

Stefan B. Schmidbauer and Oliver K. Strobel

Boehringer Mannheim GmbH, Penzberg, Germany

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
Reagents
Determination of protein concentration
Preparation of bacterial lysate
Column preparation
4. Results and discussion
5. References
6. Entire manuscript