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EXPERIMENTAL PROCEDURE FOR THE DETECTION OF A RARE HUMAN mRNA WITH THE DIG SYSTEM
Barbara Rueger, Julia Thalhammer, Irmgard Obermaier, and Stefanie Gruenewald-Janho
Boehringer Mannheim Customer Service Lab, International Support, Mannheim, Germany
4. REFERENCES
1. Schenborn, E. T. & Mierendorf, R. C. (1985) A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure. Nucl Acids Res 13(171:6223-6236).
2. Sambrook, J., Fritsch, E. M. and Maniatis, T. (1989) Molecular Cloning; A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
3. Boehringer Mannheim (1994) DIG System User's Guide for Filter Hybridization, Version 2.
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