[Frontiers in Bioscience 2, c6-8, February 15, 1997]

	[Frontiers in Bioscience 2, c6-8, February 15, 1997] Reprints PubMed CAVEAT LECTOR
	RAPID PURIFICATION OF HISTIDINE-TAGGED GLUTATHIONE S-TRANSFERASE FUSION PROTEIN BY METAL CHELATE POROS® PERFUSION CHROMATOGRAPHY® MEDIA Stefan B. Schmidbauer and Oliver K. Strobel Boehringer Mannheim GmbH, Penzberg, Germany 5. REFERENCES 1. Lindner, P., et al. (1992) Purification of Native Proteins from the Cytoplasm and Periplasm of Escherichia coli Using IMAC and Histidine Tails: A Comparison of Proteins and Protocols. METHODS: A Companion to Methods in Enzymology 4: 41-56. (No web reference available) 2. Porath, J. (1992) Immobilized Metal Ion Affinity Chromatography. Protein Expression and Purification. 3: 263-281. 3. Afeyan NB, Gordon NF, Mazsaroff I, Varady L, Fulton SP, Yang YB, Regnier FE (1990) Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: Perfusion Chromatography. J Chromatogr 519:1-29.

[Frontiers in Bioscience 2, c6-8, February 15, 1997]
Reprints
PubMed
CAVEAT LECTOR

RAPID PURIFICATION OF HISTIDINE-TAGGED GLUTATHIONE S-TRANSFERASE FUSION PROTEIN BY METAL CHELATE POROS® PERFUSION CHROMATOGRAPHY® MEDIA

Stefan B. Schmidbauer and Oliver K. Strobel

Boehringer Mannheim GmbH, Penzberg, Germany

5. REFERENCES

1. Lindner, P., et al. (1992) Purification of Native Proteins from the Cytoplasm and Periplasm of Escherichia coli Using IMAC and Histidine Tails: A Comparison of Proteins and Protocols. METHODS: A Companion to Methods in Enzymology 4: 41-56. (No web reference available)

2. Porath, J. (1992) Immobilized Metal Ion Affinity Chromatography. Protein Expression and Purification. 3: 263-281.

3. Afeyan NB, Gordon NF, Mazsaroff I, Varady L, Fulton SP, Yang YB, Regnier FE (1990) Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: Perfusion Chromatography. J Chromatogr 519:1-29.