[Frontiers in Bioscience 3, a16-22, February 15, 1998]

	[Frontiers in Bioscience 3, a16-22, February 15, 1998] Reprints PubMed CAVEAT LECTOR
	EFFECT OF TGF-BETA 1 ON PDGF RECEPTORS EXPRESSION IN HUMAN SCAR FIBROBLASTS Messadi DV^1,2 , Le A¹, Berg S¹, Huang G¹, Zhuang W¹ and Bertolami CN³ ¹School of Dentistry, ²Dental Research Institute, University of California, Los Angeles, California, ³School of Dentistry,University of California, San Francisco, California, USA Received 1/8/98 Accepted 2/3/98 7. REFERENCES 1. Kischer, C.W., Shetlar, M.R. and Chavpil, M. Hypertrophic scars and keloids: A review and new concept concerning their origin. Scan. Elect. Micros. 4: 1699-1713, (1982). 2. Border WA, Noble NA. Transforming Growth Factor beta in Tissue Fibrosis. N Engl J Med. 331: 1286-1292, (1994) 3. Heldin, C.H., Claesson-Welsh, L and Westermark, B. Platelet-derived growth factor and its receptors. In: Growth factors from genes to clinical application . By Sara VR et al eds. Raven Press New York. 41-50, (1990) 4. Grande JP. Role of Transforming growth Factor-beta in Tissue Injury and Repair. Pro Soc Exp Biol Med. 214: 27-40, (1997) 5. Bonner, J.C., Badgett, A., Lindroos, P.M., and Osornio-Vargas, A.R. Transforming Growth factor beta1 downregulates the platelet-derived growth factor alpha-receptor subtype on human lung fibroblast in vitro. Am. J. Respir. Cell Mol. Biol. 13:496-505, (1995). 6. Matsui, T., Pierce, P.H., Fleming P.T., Greenburger, J.S., LaRochelle, W.J., Ruggiero, M., and Aaronson, S.A. Independent expression of human alpha or beta platelet-derived growth factor receptor cDNAs in a naive hemtopoietic cell leads to functional coupling with mitogenic and chemotactic signaling pathways. Proc. Natl. Acad. Sci. USA, 86:8314-8318, (1989a). 7. Matsui T, Heidaran M, Miki T, Popescu N, Rochelle WL, Kraus M, Pierce J, Aaronson S: Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes. Science 243: 800-804, (1989b). 8. Seppa H, Grotendorst G, Seppa S, Schiffmann E, Martin GR: Platelet derived growth factor is chemotactic for fibroblasts. J Cell Biol 92: 584-588, (1982). 9. Battegay, E.J., Raines, E.W., Seifert, R.A., Bowen-Pope, D.F., and Ross, R. TGF-beta induces biomodal proliferation of connective tissue cells via complex control of an autocrine PDGF loop. Cell. 63:515-524, (1990). 10. Paulsson, Y., Karlsson, C., Heldin, C.H., and Westermark, B. Density-dependent inhibitory effect of transforming growth factor-beta1 on human fibroblasts involves the down-regulation of platelet-derived growth factor alpha-receptors. J. Cellular Physiology, 157:97-103,(1993). 11. Oates TW, Kose KN, Xie JF, Graves DT, Collins JM and Cochran DL. Receptor Binding of PDGF-BB, and the Modulation of PDGF Receptors by TGF-beta in Human Periodontal Ligament Fibroblasts. J Cell Physiol 162: 359-366, (1995) 12. Yamakage, A., Kikuchi, K., Smith, E.A., LeRoy, E.C., and Trojanowska, M. Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts. J. Exp. Med., 175:1227-1234 (1992). 13. Messadi DV , Sung-Cho K, Lesavoy M, Berg S and Bertolami CN. Autocrine TGF- beta 1 activity and glycosaminoglycan synthesis by human cutaneous fibroblasts. Wound Repair and Regeneration. 2: 284-291, (1994) 14. Messadi DV and Bertolami CN. CD44 and hyaluronan expression in human cutaneous scar fibroblasts. Am. J. Pathol. 142: 1041-1049, (1993). 15. Westergren-Thorsson, G., Sarnstrand, B., Fransson, L.A., Malmstrom, A. TGF-beta enhances the production of hyaluronan in human lung but not in skin fibroblasts. Exp. Cell Res. 186:192-5, (1990). 16. Chomoczymski, P and Saachi, N. Single-Step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochem. 162:156-159, (1987). 17. Chirgwin, J.A., Przybla, S., Macdonald ,R.J. and Rutter, W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochem. 18: 5294-5299, (1979). 18. Claesson-Welsh, L., Eriksson, A., Westermark, B. and Heldin, C.H. cDNA cloning and expression of the human A-type platelet derived growth factor (PDGF) receptor establishes similarity for the B-type receptor. Proc. Natl. Acad. Sci USA. 86 : 4917-4921, (1989) 19. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning a Laboratory Manual. Cold Spring Harbour Laboratory Press. Pp 7.37-7.66, (1989). 20. Wahl SH. Transforming Growth Factor beta The Good, the Bad and the Ugly. J Exp Med 180: 1587-1590, (1994). 21. Roberts, A.B. Sporn, M.B., Assoian, R. K., Smith, J.M., Roche, N.S., Wakefield, L.M., Heine, U.I., Liotta, L.A., Falanga, V., Kehrl, J.H., Fauci, A.S. Transforming growth factor beta: Rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc. Natl. Acad. Sci. USA. 83: 4167-4171, (1986). 22. Ogawa, Y., Ksander, G.A., Pratt, B.M., Sausamura, S.J., Zunian, J.M., Gerhardt, C.O., Avis, P.D., Murray, M.J. and McPherson, J. Differences in the Biological activities of Transforming growth factor-beta and Platelet derived growth factor in vivo. Growth Factors 5: 57-68, (1991) 23. Mustoe, T.A., Pierce, G.F., Thomason, A., Gramates, P., Sporn, M.B and Deuel, T.F. Accelerated healing of insicional wounds in rats induced by transforming growth factor type beta. Science 237: 1333-1336, (1987). 24. Ksander, G.A., Ogawa, Y., Chu, G.H., McMullin, H., Rosenblatt, J.S., McPherson J.M. Exogenous transforming growth factor-beta 2 enhances granulation tissue formation and wound strength in guinea pig dermal wounds healing by secondary intent. Ann. Surg. 211: 288-294, (1990a). 25. Ksander, G.A., Chu, G.H., McMullin, H., Ogawa, Y., Pratt, B.M., Rosenblatt, J.S. and McPherson, J.M. Transforming growth factor beta1 and beta2 enhance connective tissue formation in animal models of dermal wound healing by secondary intent. Ann. N.Y Acad. Sci. 593: 135-147, (1990b). 26. Ichiki, Y., Smith, E., LeRoy, E.C., Trojanowska, M. Different effects of basic fibroblast growth factor and transforming growth factor-beta on the two platelet-derived growth factor receptors' expression in scleroderma and healthy human dermal fibroblasts. J. Invest. Dermatol., 104:124-127, (1995). 27. Kikuchi K, Kadano T, Ihn H, Sato S, Igarashi A, Nakagawa H, Tamaki K and Takehara K. Growth Regulation in Scleroderma Fibroblasts: Increased Response to Transforming Growth Factor- beta 1. J Invest Dermatol 105: 128-132, (1995). 28. Krane JF, Murphy DP, Gottlieb AB, Carter DM, Hart CE, Krueger JG: Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis. J Invest Dermatol 96: 983-986, (1991). 29. Reuterdahl C, Sundberg C, Rubin K, Funa K, Gerdin B: Tissue localization of beta receptors for platelet-derived growth factor and platelet derived growth factor B chain during wound repair in humans. J Clin Invest 91: 2065-2075, (1993) 30. Klareskog L, Gustafsson R, Scheynius A, Hallgren R: Increased expression of platelet derived growth factor type Beta receptors in the skin of patients with systemic sclerosis. Arthr and Rheum 33: 1534-1541, (1990) 31. Haisa M, Okochi H, Grotendorst GR. Elevated Levels of PDGF alpha Receptors in Keloid Fibroblasts Contribute to an Enhanced Response to PDGF. J Invest Dermatol. 103: 560-563, (1994). 32. Clark, R.A.F., McCoy, G.A., Folkvord, J.M., and McPherson, J.M. TGF-beta1 stimulates cultured human fibroblasts to proliferate and produce tissue-like fibroplasia: A fibronectin matrix-dependent event. J. Cellular Physiology. 170:69-80, . (1997)

[Frontiers in Bioscience 3, a16-22, February 15, 1998]
Reprints
PubMed
CAVEAT LECTOR

EFFECT OF TGF-BETA 1 ON PDGF RECEPTORS EXPRESSION IN HUMAN SCAR FIBROBLASTS

Messadi DV^1,2 , Le A¹, Berg S¹, Huang G¹, Zhuang W¹ and Bertolami CN³

¹School of Dentistry, ²Dental Research Institute, University of California, Los Angeles, California, ³School of Dentistry,University of California, San Francisco, California, USA

Received 1/8/98 Accepted 2/3/98

7. REFERENCES

1. Kischer, C.W., Shetlar, M.R. and Chavpil, M. Hypertrophic scars and keloids: A review and new concept concerning their origin. Scan. Elect. Micros. 4: 1699-1713, (1982).

2. Border WA, Noble NA. Transforming Growth Factor beta in Tissue Fibrosis. N Engl J Med. 331: 1286-1292, (1994)

3. Heldin, C.H., Claesson-Welsh, L and Westermark, B. Platelet-derived growth factor and its receptors. In: Growth factors from genes to clinical application . By Sara VR et al eds. Raven Press New York. 41-50, (1990)

4. Grande JP. Role of Transforming growth Factor-beta in Tissue Injury and Repair. Pro Soc Exp Biol Med. 214: 27-40, (1997)

5. Bonner, J.C., Badgett, A., Lindroos, P.M., and Osornio-Vargas, A.R. Transforming Growth factor beta1 downregulates the platelet-derived growth factor alpha-receptor subtype on human lung fibroblast in vitro. Am. J. Respir. Cell Mol. Biol. 13:496-505, (1995).

6. Matsui, T., Pierce, P.H., Fleming P.T., Greenburger, J.S., LaRochelle, W.J., Ruggiero, M., and Aaronson, S.A. Independent expression of human alpha or beta platelet-derived growth factor receptor cDNAs in a naive hemtopoietic cell leads to functional coupling with mitogenic and chemotactic signaling pathways. Proc. Natl. Acad. Sci. USA, 86:8314-8318, (1989a).

7. Matsui T, Heidaran M, Miki T, Popescu N, Rochelle WL, Kraus M, Pierce J, Aaronson S: Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes. Science 243: 800-804, (1989b).

8. Seppa H, Grotendorst G, Seppa S, Schiffmann E, Martin GR: Platelet derived growth factor is chemotactic for fibroblasts. J Cell Biol 92: 584-588, (1982).

9. Battegay, E.J., Raines, E.W., Seifert, R.A., Bowen-Pope, D.F., and Ross, R. TGF-beta induces biomodal proliferation of connective tissue cells via complex control of an autocrine PDGF loop. Cell. 63:515-524, (1990).

10. Paulsson, Y., Karlsson, C., Heldin, C.H., and Westermark, B. Density-dependent inhibitory effect of transforming growth factor-beta1 on human fibroblasts involves the down-regulation of platelet-derived growth factor alpha-receptors. J. Cellular Physiology, 157:97-103,(1993).

11. Oates TW, Kose KN, Xie JF, Graves DT, Collins JM and Cochran DL. Receptor Binding of PDGF-BB, and the Modulation of PDGF Receptors by TGF-beta in Human Periodontal Ligament Fibroblasts. J Cell Physiol 162: 359-366, (1995)

12. Yamakage, A., Kikuchi, K., Smith, E.A., LeRoy, E.C., and Trojanowska, M. Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts. J. Exp. Med., 175:1227-1234 (1992).

13. Messadi DV , Sung-Cho K, Lesavoy M, Berg S and Bertolami CN. Autocrine TGF- beta 1 activity and glycosaminoglycan synthesis by human cutaneous fibroblasts. Wound Repair and Regeneration. 2: 284-291, (1994)

14. Messadi DV and Bertolami CN. CD44 and hyaluronan expression in human cutaneous scar fibroblasts. Am. J. Pathol. 142: 1041-1049, (1993).

15. Westergren-Thorsson, G., Sarnstrand, B., Fransson, L.A., Malmstrom, A. TGF-beta enhances the production of hyaluronan in human lung but not in skin fibroblasts. Exp. Cell Res. 186:192-5, (1990).

16. Chomoczymski, P and Saachi, N. Single-Step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochem. 162:156-159, (1987).

17. Chirgwin, J.A., Przybla, S., Macdonald ,R.J. and Rutter, W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochem. 18: 5294-5299, (1979).

18. Claesson-Welsh, L., Eriksson, A., Westermark, B. and Heldin, C.H. cDNA cloning and expression of the human A-type platelet derived growth factor (PDGF) receptor establishes similarity for the B-type receptor. Proc. Natl. Acad. Sci USA. 86 : 4917-4921, (1989)

19. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning a Laboratory Manual. Cold Spring Harbour Laboratory Press. Pp 7.37-7.66, (1989).

20. Wahl SH. Transforming Growth Factor beta The Good, the Bad and the Ugly. J Exp Med 180: 1587-1590, (1994).

21. Roberts, A.B. Sporn, M.B., Assoian, R. K., Smith, J.M., Roche, N.S., Wakefield, L.M., Heine, U.I., Liotta, L.A., Falanga, V., Kehrl, J.H., Fauci, A.S. Transforming growth factor beta: Rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc. Natl. Acad. Sci. USA. 83: 4167-4171, (1986).

22. Ogawa, Y., Ksander, G.A., Pratt, B.M., Sausamura, S.J., Zunian, J.M., Gerhardt, C.O., Avis, P.D., Murray, M.J. and McPherson, J. Differences in the Biological activities of Transforming growth factor-beta and Platelet derived growth factor in vivo. Growth Factors 5: 57-68, (1991)

23. Mustoe, T.A., Pierce, G.F., Thomason, A., Gramates, P., Sporn, M.B and Deuel, T.F. Accelerated healing of insicional wounds in rats induced by transforming growth factor type beta. Science 237: 1333-1336, (1987).

24. Ksander, G.A., Ogawa, Y., Chu, G.H., McMullin, H., Rosenblatt, J.S., McPherson J.M. Exogenous transforming growth factor-beta 2 enhances granulation tissue formation and wound strength in guinea pig dermal wounds healing by secondary intent. Ann. Surg. 211: 288-294, (1990a).

25. Ksander, G.A., Chu, G.H., McMullin, H., Ogawa, Y., Pratt, B.M., Rosenblatt, J.S. and McPherson, J.M. Transforming growth factor beta1 and beta2 enhance connective tissue formation in animal models of dermal wound healing by secondary intent. Ann. N.Y Acad. Sci. 593: 135-147, (1990b).

26. Ichiki, Y., Smith, E., LeRoy, E.C., Trojanowska, M. Different effects of basic fibroblast growth factor and transforming growth factor-beta on the two platelet-derived growth factor receptors' expression in scleroderma and healthy human dermal fibroblasts. J. Invest. Dermatol., 104:124-127, (1995).

27. Kikuchi K, Kadano T, Ihn H, Sato S, Igarashi A, Nakagawa H, Tamaki K and Takehara K. Growth Regulation in Scleroderma Fibroblasts: Increased Response to Transforming Growth Factor- beta 1. J Invest Dermatol 105: 128-132, (1995).

28. Krane JF, Murphy DP, Gottlieb AB, Carter DM, Hart CE, Krueger JG: Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis. J Invest Dermatol 96: 983-986, (1991).

29. Reuterdahl C, Sundberg C, Rubin K, Funa K, Gerdin B: Tissue localization of beta receptors for platelet-derived growth factor and platelet derived growth factor B chain during wound repair in humans. J Clin Invest 91: 2065-2075, (1993)

30. Klareskog L, Gustafsson R, Scheynius A, Hallgren R: Increased expression of platelet derived growth factor type Beta receptors in the skin of patients with systemic sclerosis. Arthr and Rheum 33: 1534-1541, (1990)

31. Haisa M, Okochi H, Grotendorst GR. Elevated Levels of PDGF alpha Receptors in Keloid Fibroblasts Contribute to an Enhanced Response to PDGF. J Invest Dermatol. 103: 560-563, (1994).

32. Clark, R.A.F., McCoy, G.A., Folkvord, J.M., and McPherson, J.M. TGF-beta1 stimulates cultured human fibroblasts to proliferate and produce tissue-like fibroplasia: A fibronectin matrix-dependent event. J. Cellular Physiology. 170:69-80, . (1997)