[Frontiers in Bioscience 3, a16-22, February 15, 1998]
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EFFECT OF TGF-BETA 1 ON PDGF RECEPTORS EXPRESSION IN HUMAN SCAR FIBROBLASTS

Messadi DV1,2 , Le A1, Berg S1, Huang G1, Zhuang W1 and Bertolami CN3

1 School of Dentistry, 2 Dental Research Institute, University of California, Los Angeles, California, 3 School of Dentistry,University of California, San Francisco, California, USA

Received 1/8/98 Accepted 2/3/98

TABLE OF CONTENTS


1. Abstract
2. Introduction
3. Materials and Methods
3.1 Cell cultures
3.2 Addition of exogenous TGF- beta 1
3.3 Radioimmunobinding assay
3.4 RNA extraction
3.5 Polymerase chain reaction amplification
3.6 Northern blot analysis
3.7 Western blot analysis
4. Results
4.1. Effect of TGF-beta 1 on PDGF receptors subunits: RadioImmunoBinding assay
4.2 TGF-beta 1 Modulation of PDGF-alpha and betareceptor Protein Synthesis
4.3 TGF-beta 1 Effect on PDGF-alpha and beta receptor mRNA Expression
5. Discussion
6. Acknowledgement
7. References
8. Entire manuscript

Key words: Dermal fibroblasts, Keloid, Growth factors

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