[Frontiers in Bioscience 3, a11-15, February 1, 1998]
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CAVEAT LECTOR




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TARGETED INHIBITION OF HEPATITIS B VIRUS GENE EXPRESSION: A GENE THERAPY APPROACH

Frank Y.-T. Tung and Steven W. Bowen

Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh PA 15261

Received 1/21/98 Accepted 1/28/98

TABLE OF CONTENTS


1. Abstract
2. Introduction
3. Materials and methods
3.1. Construction of retroviral vectors and producer lines
3.2. Transient assay of antisense-mediated inhibition of HBsAg
3.3. Northern blot analysis of antisense and sense RNA expressed in transduced HepG2 cells

4. Results
4.1 construction of retroviral vectors and producer lines encoding an antisense and sense copy of surface antigen.
4.2 inhibition of HBAg production by co-transfection assay
4.3 inhibition of HBsAg production in the antisense-transduced HepG2 cells

5. Discussion
6. Acknowledgment
7. References
8. Entire manuscript

Key words: Hepatitis B Virus, Surface antigen, Gene therapy, Chronic hepatitis, Retroviral vector.