EARLY INDUCTION AND AUGMENTATION OF PARASITIC ANTIGEN-SPECIFIC ANTIBODY-PRODUCING B LYMPHOCYTES IN THE NON-PEYER’S PATCH REGION OF THE SMALL INTESTINE

Ching-Hua Wang¹, Elizabeth M. Richards¹, Robert D. Block², Enio M. Lezcano¹ and Ricardo Gutierrez¹

1 Biology Department, California State university, 5500 University parkway, San Bernardino, CA 92407, USA, ² Department of Neurology and Neural Science, School of Medicine, University of Southern California, Los Angeles, CA 90033, USA

Received 10/12/98 Accepted 10/20/98

TABLE OF CONTENTS

1. Abstract

2. Introduction

3. Materials and methods

3.1. Animals
3.2. Parasite
3.3. Collection of muscle larvae
3.4. Preparation of adult antigens
3.5. Infection with muscle larvae
3.6. Obtaining tissues and cryohistological preparation
3.7. Immunofluorescence assay (IFA)
3.8. Statistical analysis

4. Results

4.1. Kinetics of appearance of 9D4 antigen-specific IgM-producing B lymphocytes
4.2. Kinetics of appearance of 9D4 antigen-specific IgA-producing B lymphocytes
4.3. Kinetics of appearance of 9D4 antigen-specific IgE-producing B lymphocytes
4.4. Kinetics of appearance of 9D4 antigen-specific IgG1-producing B lymphocytes
4.5. Kinetics of appearance of 9D4 antigen-specific IgG2a-producing B lymphocytes
4.6. Kinetics of appearance of 9D4 antigen-specific IgG2b-producing B lymphocytes
4.7. Kinetics of appearance of 9D4 antigen-specific IgG2c-producing B lymphocytes

5. Discussion

6. Acknowledgment

7. References

8. Entire manuscript