William Y. Sun and Sandra J. F. Degen

Division of Developmental Biology, Children's Hospital Research Foundation, Cincinnati, OH 45229

TABLES

Table 1. Properties of Prothrombin

Functions	Substrates or target cells
Coagulation	Fibrinogen
	Factor XIII
	Platelets (PARs and integrin aIIbb3)
Anticoagulation	Protein C
	Factors Va and VIIIa
Inflammation	Monocytes
	Macrophages
Atherosclerosis/restenosis	Vascular smooth muscle cells
	Endothelial cells
CNS functions/response to injury	Neurons
	Astrocytes
	Synapses
Tissue repair	Fibroblasts
	Endothelial cells
Mitogen	Fibroblasts
	Endothelial cells
	Astrocytes
	Vascular smooth muscle cells

Table 2. Comparison of the lengths of exons and introns in the genes coding for human, bovine and mouse prothrombin¹

Exon	Human amino acids	Human Gene2 (bp)	Bovine Gene 3 (bp)	Mouse Gene (bp)	Intron	Human Gene2 (bp)	Bovine Gene 3 (bp)	Mouse Gene (bp)
1	-43 to -17	79+4	94+5	79+4	A	386	342	376
2	-17 to 37	161	164	164	B	659	601+/- 62	579
3	38-46	25	25	25	C	242	227	221
4	46-63	51	51	51	D	2326	1504+/-73	1600
5	63-98	106	106	106	E	96	98	78
6	98-144	137	137	137	F	2338	1381+/-99	2146
7	144-249	315	315	318	G	324	293	242
8	249-292	129	135	114	H	84	75	76
9	292-334	127	127	127	I	1157	1055+/-94	631
10	334-390	168	168	168	J	497	397+/-46	317
11	390-448	174	174	ND	K	542	242	ND
12	448-509	182	182	ND	L	9447	6940+/-255	ND
13	509-532	71	71	ND	M	146	135	128
14	533-polyA	241	266	253

ND: A portion of the mouse prothrombin gene was not isolated and therefore not characterized; this included exons 11-13 and intervening sequences K and L. ¹ All sizes were determined by DNA sequence analysis, except for those in the bovine gene indicated with standard deviations which were determined by heteroduplex analysis with the electron microscope. ² Reference 93 ³ Reference 95 ⁴ Due to variability in the transciption start site the size of the exon is indicated as starting at the initiator methionine ⁵ The length of exon 1 is indicated as the size starting in the 5' flanking region where the longest cDNA started.

Table 3. Exon-intron junctions in the mouse prothrombin gene¹

E1

A

T

G

G

T

A

A

G

G

C

T

G

C

A

G

T

G

T

E2

E2

A

C

A

G

T

G

A

G

T

T

T

G

T

A

G

G

A

C

E3

E3

C

A

G

G

T

G

A

G

T

T

T

T

T

A

G

G

A

C

E4

E4

A

A

G

G

T

G

G

G

G

C

T

G

T

A

G

G

T

C

E5

E5

T

G

A

G

T

G

A

G

T

T

T

A

C

A

G

A

A

T

E6

E6

G

T

G

G

T

G

A

G

C

C

A

C

C

A

G

G

C

C

E7

E7

G

C

G

G

T

G

A

G

C

C

T

A

C

A

G

A

G

G

E8

E8

C

A

G

G

T

G

A

G

A

C

T

G

C

A

G

A

C

T

E9

E9

C

T

G

G

T

G

C

G

T

C

G

T

T

A

G

G

C

A

E10

E10

C

A

G

G

T

A

T

G

A

G

C

A

C

A

G

A

T

A

E11

E11

E12

E12

E13

E13

A

A

G

G

T

A

T

G

C

C

C

T

C

A

G

A

G

C

E14

1Exons 11 and 12 and the corresponding intervening sequences have not been isolated

Table 4. Genotypes of embryos and pups from matings of mice hemizygous for the targeted prothrombin allele (FII+/- x FII+/-)

Prothrombin Genotype
Stage	Total	Genotyped	+/+	+/-	-/-	%1
E8.5	37	34	10	16	8	23.5
E9.5	76	73	15	39	18	25.4
E10.5	104	103	29	58	7	6.8
E11.5	36	32	6	19	3	9.4
E12.5	99	86	26	46	8	9.3
E15.5	53	48	19	24	5	10.4
E18.5	96	95	34	51	10	10.5
Term	135	134	47	77	10	7.4

1Viable prothrombin-deficient mice calculated as the percentage of all genotyped mice in the same age group.