[Frontiers in Bioscience 8, a79-84, May 1, 2003]

MEMBRANE AND SYNAPTIC EFFECTS OF ANTI-S-100 ARE PREVENTED BY THE SAME ANTIBODIES IN LOW CONCENTRATIONS

O.I. Epstein 1, N.A. Beregovoy 2, N.S. Sorokina 2, M.V. Starostina 2, M.B. Shtark 2, Kh. L. Gainutdinov 3, T. Kh. Gainutdinova 3, D.I. Muhamedshina 3

1 "Materia Medica Holding" Research and Production Company, Moscow 2 Institute of Molecular Biology and Biophysics, Siberian Department of Russian Academy of Medical Sciences, Novosibirsk;; 3 Kazan Physicotechnical Institute, Kazan, Russia

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and Methods
3.1. Experimental and control serum
3.2. Experiments on identified neurons from subesophageal complex of ganglia of terrestrial snails
3.3. Long-term potentiation (LTP) in rat hippocampal slices
4. Results
4.1. The effects of antibodies to S-100 in "high" and low concentrations on electrical characteristics of snail identified neurons
4.2. Effects of antibodies to S-100 protein in "high" and low concentrations on LTP formation in rat hippocampal slices
5. Discussion
6. References

1. ABSTRACT

Effects of antiserum to S-100 protein (AS-100) in high (HC, corresponding to antiserum dilution 1:5 - 1:50) and low (LC, 1:1012) concentrations were studied in identified snail neurons and rat hippocampal slices. HC-AS-100 changed the frequency of action potential generation in spontaneously active neurons and blocked formation of long-term potentiation in mossy fiber synapses. LC-AS-100 alone did not affect any characteristic measured in our experiments, but 20 min pre-incubation of snail ganglia or hippocampal slices with LC-AS-100 abolished the effects of the same antibodies in HC. Simultaneous addition of both LC and HC did not prevent the development of HC-AS-100 effects. It seems possible that LC-AS-100 is capable of interaction with neuronal cells modifying their reaction to HC-AS-100.