Cristian Apetrei ¹, David L. Robertson² and Preston A. Marx ¹

1 Microbiology Division, Tulane National Primate Research Center, Covington, LA, ² School of Biological Sciences, University of Manchester, United Kingdom

TABLES

Table 1. African non-human primates infected with SIV

		Viral type	Full-length genome availability	Partial sequence	Serological evidence7	Pathogenic in host species8	Pathogenic in heterologous hosts
Humans	Homo sapiens	HIV-1 group M	M62320, K03455, U211353	Yes		Y	N
		HIV-1 group O	L20587, L20571	Yes		Y	NA
		HIV-1 group N	AJ271370, AJ006022	Yes		Y	NA
		HIV-2 group A	X05291, NC001722	Yes		Y	Y
		HIV-2 group B	U27200, X61240	Yes		Y	Y
Non-human primates
Chimpanzees	Pan troglodytes troglodytes (Central African chimp)	SIVcpzGAB/ CAM/US	X52154, AF103818, AF115393, AJ271369	U114956		N	Y
	P. t. schweinfurthii (Eastern chimp)	SIVcpzANT/TAN	U42720, AF447763	Yes		N	NA
	P. t. vellorosus	SIVcpzCAM41		AF115394		N	NA
Cercopithecoidea
Cercopithecinae
Papionini
Mangabeys	Cerccebus atys (sooty mangabey)	SIVsm	X14307, U72748, M80194, AF077017, AF334679	X78505-11, AY158968-84		N	Y
	Cercocebus torquatus (red-capped mangabey)	SIVrcm	AF349680	AF349681, AF028607, AF028608		N	N
	Cercocebus agilis (agile mangabey)			Yes		N	NA
	Cercocebus torquatus lunulatus (white-crowned mangabey)	SIVagm.Ver1	NA4	D10702
	Lophocebus albigena (gray-crested mangabey)				Yes
Mandrills	Mandrillus sphinx (mandrill)	SIVmnd-1	M27470	AF328282, AF328283		Y	NA
		SIVmnd-2	AF328295, AY159322			Y	N
	M. leucophaeus (drill)	SIVdrl	AY159321	AJ011017		N	NA
Baboons	Papio cynocephalus (yellow baboon)	SIVagm.Ver2	NA	Yes	Yes	NR9
	Papio ursinus (chacma baboon)	SIVagm.Ver2	NA	Yes	Yes	NR
Macaques	Macacca mulatta (rhesus macaque)	SIVmac1	M19499, D01065, M76764, M16403			Y
	M. nemestrina (pig-tailed macaque)	SIVmne1	M32741, U79412			Y
	M. arctoides (stump-tailed macaque)	SIVstm1	M83293			Y
Cercopithecini
Allenopithecus	A. nigroviridis (Allen's monkey)				Yes
Talapoins	Miopithecus talapoin (talapoin)	SIVtal	NA	AF119357-58		N	NA
	M. ougouensis (Gabon talapoin)	SIVtal	NA	AF478594-95
Patas	Erythrocebus patas	SIVagm.Sab2	NA				NA
African greens	Chlorocebus aethiops (grivet)	SIVagm.Gri	M66437	Yes		N	NA
	C. pygerythrus (vervet)	SIVagm.Ver	X07805, M29975 M30931	Yes		Y	Y
	C. tantalus (tantalus)	SIVagm.Tan	U58991	Yes		N	NA
	C. sabaeus (sabaeus)	SIVagm.Sab	U04005	Yes		N	N
Cercopithecus genus
Diana group	C. diana (diana monkey)		NA	NA
Mitis group	C. nictitans (greater spot-nosed monkey)	SIVgsn	AF468658, AF468659	AF478588-90		NR	NA
	C. mitis (blue monkey)	SIVblu	NA	Yes		NR	NA
	C. albogularis (Syke's monkey)	SIVsyk	L06042			NR	NA
Mona group	C. mona (mona monkey)	SIVmon	AJ549283	AF478591		NR	NA
	C. denti (Dent's mona)	SIVden	Yes5	Yes		NR	NA
	C. pogonias (crested mona)				Yes
	C. lowei (Lowe's mona)				Yes
Cephus group	C. cephus (mustached guenon)	SIVmus	Yes	AF478592-93		NR	NA
	C. ascanius (red-tailed monkey)	SIVasc	Yes			NR	NA
Lhoesti group	C. lhoesti (L'Hoest's monkey)	SIVlhoest	AF075269, AF188114, AF188115, AF188116			NR	Y
	C. solatus (sun-tailed monkey)	SIVsun	AF131870			NR	NA
Neglectus group	C. neglectus (De Brazza's monkey)	SIVdeb	Yes	AJ549756, AF478600-05		NR	NA
Hamlyni group	C. hamlyni (Owl-faced monkey)				Yes
Colobinae	Colobus guereza (C. guereza)	SIVcol	AF301156	AF301154, AF301155		NR	NA
	Piliocolobus badius (western red colobus)	SIVwrc	NA	AY138265-68		NR	NA
	Procolobus verus (olive colobus)	SIVolc	NA	AY138269		NR	NA

1Accidentally transmitted in captivity ² Naturally occurring in the wild ³ GeneBank accession numbers of the full-length sequences. For HIVs, only some reference strains out of more than 150full-length genomic sequences available ⁴ NA-not available ⁵ Some full-length sequences are not yet released ⁶ For some SIVs, only partial genomic sequences are available. In those instances in which full-length sequences are available for a given virus, some partial sequences of interest are also listed in this column ⁷ Only serological evidence reported for these viruses ⁸ Although most reports suggest that SIVs are not pathogenic in their natural non-human primate hosts, cases of immunesuppression have been reported ⁹Not reported

Table 2. Primer sets used for "diagnostic" PCR to identify divergent SIVs

Primer name	Position	Primer sequence	Reference
UNIPOL 1	2420	AGTGGATTCATAGAAGCAGAAGT	83
UNIPOL 2	2700	CCCCTATTCCTCCCCTTCTTTTAAAA	83
UNIPOL 3	1940	TGTCAACATAGTAACAGATTCACAATA	83
UNIPOL 4	2900	ACTACTGCCCCTTCACCTTTCCA	83
HPOL4538	2900	TACTGCCCCTTCACCTTTCCA	84
HPOL4235	2570	CCCTACAATCCCCAAAGTCAAGG	84
HPOL4327	2650	TAAGACAGCAGTACAAATGGCAG	84
HPOL4481	2800	GCTGTCCCTGTAATAAACCCG	84
DR1	235	TRCAYACAGGRGCWGAYGA	77
DR2	1020	AIADRTCATCCATRTAYTG	77
DR4	710	GGIATWCCICAYCCDGCAGG	77
DR5	930	GGIGAYCCYTTCCAYCCYTGHGG	77
Polis4	2090	CCAGCNCACAAAGGNATAGGAGG	69
PolOR	2902	ACBACYGCNCCTTCHCCTTTC	69

Different primer combinations see Figure 1 may be used

Table 3. Host range of SIVs in different cell lines

Growth support

Cell description

SIVcpz

SIVsm

SIVmac

SIVagm

SIVlhoest

SIVsun

SIVmnd-1

SIVsyk

SIVtal

SIVrcm

SIVmnd-2

SIVdrl

human PBMC1

+

+

+

±

+

-

+

-

-

+

+

+

human MDM2

-

+

+

±

+

-

-

-

±

macaque PBMC

+

+

-

-

+

+

chimpanzee PBMC

+

-

+

+

-

-

-

-

-

MT2

T cell line

+

-

-

-

-

-

-

C8166

T cell line

+

+

+

-

-

-

H9

Cloned from Hut78

+

+

-

-

-

+

-

-

MT4

T cell line

+

+

+

+

-

-

+

U937

Promonocytic cell line

-

-

-

-

-

-

-

-

SupT1

T cell line

-

+

+

+

+

-

-

+

+

PM1

-

-

-

+

-

-

Hut78

T cell line

+

+

-

-

-

-

-

Molt 4 Clone 8

T cell line

+

-

-

+

+

+

-

-

-

+

+

CEMss

T cell line

+

+

+

+

-

-

+

CEMx174

T cell - B cell hybrid line

+

+

-

+

-

++

+

-

+

+