[Frontiers in Bioscience 9, 110-116, January 1, 2004]

OXYGEN SENSORS AND ENERGY SENSORS ACT SYNERGISTICALLY TO ACHIEVE A GRADED ALTERATION IN GENE EXPRESSION: CONSEQUENCES FOR ASSESSING THE LEVEL OF NEUROPROTECTION IN RESPONSE TO STRESSORS

Gillian M.C. Renshaw 1, Joshua Warburton 2 and Adeeb Girjes 3

1 School of Physiotherapy and Exercise Science, Griffith University, Gold Coast Campus, PMB 50 Gold Coast Mail Centre, 9726 Queensland, Australia. 2 Department of Anatomical and Developmental Biology, University of Queensland, St Lucia, 4072 Queensland, Australia. 3 Department of Zoology and Entomology, University of Queensland, St Lucia, 4072 Queensland, Australia

FIGURES

Figure 1. (A) Shows the western blot of Hsp70 protein bands for the brainstem from hypoxia-preconditioned animals and their pair matched controls. Hela cells were used as a positive control for Hsp70. The 67Kda molecular weight marker appears in the last column. (B) Shows the absorbance measure for each of the brainstems. Abbreviations: the series denoted by C refers to brainstems from control animals exposed to normoxia and the series denoted by E refers to brainstems from hypoxia-preconditioned animals.

Figure 2. (A) Shows the western blot of Hsp70 protein bands for the cerebella from hypoxia-preconditioned animals and their pair matched controls. Hela cells were used as a positive control for Hsp70. The 67Kda molecular weight marker appears in the last column. (B) Shows the absorbance measure for each of the cerebella. Abbreviations: the series denoted by C refers to the cerebella from control animals exposed to normoxia and the series denoted by E refers to the cerebella from anoxia-preconditioned animals.

Figure 3. (A) Shows the western blot of Hsp70 protein bands for the cerebella from normoxic untreated animals and pair matched anoxia-preconditioned animals treated either with aminophylline or saline prior to their final anoxic episode. Hela cells were used as a positive control for Hsp70. The 67Kda molecular weight marker appears in the last column. (B) Shows the absorbance measure for each of the cerebella. Abbreviations: the series denoted by C refers to the cerebella from control animals exposed to normoxia, the series denoted by A refers to the cerebella from anoxia-preconditioned, aminophylline treated animals and the series denoted by S refers to the cerebella from anoxia-preconditioned, saline treated animals.