[Frontiers in Bioscience 10, 2412-2419, September 1, 2005]

INTRON POSITION CONSERVATION ACROSS EUKARYOTIC LINEAGES IN TUBULIN GENES


Bagavathi S. Perumal 1, Kishore R. Sakharkar 2
, Vincent Tak Kwong Chow 3, Pandjassarame Kangueane 1, and Meena Kishore Sakharkar 1

1 NCSV, MAE, Nanyang Technological University, Singapore 639798, 2 BioInformatics Institute, 30 Biopolis Street, #07-01, Matrix, Singapore 138671, 3Department of Microbiology, National University of Singapore, Singapore 119260

TABLE OF CONTENTS

Abstract
2. Introduction
3. Materials and methods
4. Results
5. Discussion
6. Conclusion
7. Acknowledgements
8. References

1. ABSTRACT

A compilation of intron positions obtained from a large number of eukaryotic genomes across orthologous tubulins is explored for molecular evolution. Comparison of intron positions for 41 α, 80 β, and 30 γ tubulin genomic sequences indicates that the putative ancestral tubulin gene contained at least 19, 33, and 52 intron positions distributed at different sites in the coding regions for α, β, and γ tubulins, respectively. Many intron positions are old and are conserved across different eukaryotic lineages and intron distribution patterns are consistent with 'introns-early' hypothesis.