[Frontiers in Bioscience 11, 186-197, January 1, 2006]

Novel probes for protein chip applications

Wei Liao, Su Guo, and Xin Sheng Zhao

State Key Laboratory of Molecular Dynamic and Stable Structures, and Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, China


1. Abstract
2. Introduction
3. Phage antibody chip
3.1. Phage display
3.2. Immobilization of phage on surfaces
3.3. Application of phage antibody chip
4. Protein-oligonucleotide conjugates (POC)
4.1. Background
4.2. Fabrication of POC
4.3. Microarrays by POC
4.4. Active controlled chip
5. Aptamer
5.1. Introduction to aptamer
5.2. Aptamer microarrays
6. Perspective
7. Acknowledgments
8. References


Protein microarrays (protein chips), due to their high throughput, have great potential to reduce the cycle time of drug discovery and to improve the efficiency of medical diagnostics. A great deal of effort has been made to facilitate the development of protein chips, such as the antibody microarrays. High-throughput fabrication of purified high-affinity probes is now one of the bottlenecks for protein microarrays. We discuss here three novel strategies to fabricate probes, namely, single chain antibodies displayed by phage, protein-oligonucleotide conjugates, and aptamers, which are promising in the protein chip technology.