[Frontiers in Bioscience 13, 842-855, January 1, 2008]

Homologous housekeeping proteins in Nocardia - the NoDaMS proteomic database

Simone Koenig1, Anna-Maria Mehlich1, Doreen Ackermann1, Mohammed E. Hamid2, Nageeb S. Saeed3, Moawia Mukhtar4, Mogahid El Hassan5

1Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, University of Muenster, Germany, 2Department of Microbiology, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia, 3General Directorate of Laboratories and Medical Research, Federal Ministry of Health, Sudan, 4General Directorate of the Institute of Endemic Diseases, University of Khartoum, Sudan, 5College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Experimental considerations
3.1. Strains
3.2. Protein purification and analysis
4. Proteomic data
5. Abundant Nocardia proteins
6. Conclusion
7. Acknowledgement
8. References

1. ABSTRACT

Nocardiosis is on the rise but hard to diagnose and the application of advanced subtyping technologies is called for. While the genomic sequence for the most virulent strain, Nocardia farcinica is available, proteome data are essentially non-existent. Nevertheless, they are necessary for functional studies on virulence and disease prevention. Here, comparative gel electrophoresis (PAGE)-based analyses of the five Nocardia strains SD1828, N. africana SD910, SD 925, N. sp. 1086, and N. asteroides N317 are discussed. The two-dimensional gel images of all strains are similar and dominated by housekeeping proteins such as chaperones and metabolic enzymes. The sequences of many proteins are highly homologous among strains and in some cases Mycobacterium sequences are closer matches to the unknown than those of N. farcinica. All mass spectrometry data are made available in the NoDaMS database at URL http://ifg.uni-muenster.de/ (Proteomics-Projects-NoDaMS) for re-evaluation with fresh sequencing information. Assignments, homology analyses, and peptide matches are presented. This data review comprises the first comprehensive summary of proteomic data of Nocardia.