[Frontiers in Bioscience 14, 59-71, January 1, 2009]

Genetic engineering of plant food with reduced allergenicity

Stephan Scheurer1, Sophia Sonnewald2

1Paul-Ehrlich-Institut, Division Allergology, Paul-Ehrlich-Str. 51-59, Langen, Germany, 2Friedrich-Alexander University Erlangen-Nueremberg, Staudtstr. 5, Erlangen, Germany

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Reduction of plant food allergens by RNA interference
3.1. Genetic engineering / RNA silencing in plants
3.2. Suppression of allergens in plant food by gene silencing
3.3. Potential and limitations of RNAi mediated suppression of allergens in plant food
3.4. Risk assessment of genetically modified foods
3.5. Alternative strategies by non-GM techniques
4. Summary and perspective
5. Acknowledgements
6. References

1. ABSTRACT

Food allergies are a major health concern in industrialized countries. Since a specific immunotherapy for food allergies is not available in clinical routine praxis till now, reduction of allergens in foods, either by food processing or genetic engineering are strategies to minimize the risk of adverse reactions for food allergic patients. This review summarizes biotechnological approaches, especially the RNA interference (RNAi) technology, for the reduction of selected allergens in plant foods. So far, only a limited number of reports showing proof-of-concept of this methodology are available. Using RNAi an impressive reduction of allergen accumulation was obtained which was stable in the next generations of plants. Since threshold doses for most food allergens are not known, the beneficial effect has to be evaluated by oral challenge tests in the future. The article critically addresses the potential and limitations of genetic engineering, as well as of alternative strategies to generate "low allergic" foods.