Tanya Johnson^1,2,7, Allal Ouhtit ^3,7, Rajiv Gaur^3,7, Augusta Fernando^3,7, Paul Schwarzenberger^4,7, Joseph Su^5,7, Mohamed F Ismail⁸, Hassan I El-Sayyad⁸,Anjali Karande⁶, Zakaria Abd Elmageed^3,7, Prakash Rao⁷, Madhwa Raj^1,2,7

1Department of Biochemistry, Louisiana State University Health Sciences Center, New Orleans, LA, ²Department of Obstetrics-Gynecology, Louisiana State University Health Sciences Center, New Orleans, LA, ³Department of Pathology, Louisiana State University Health Sciences Center, New Orleans, LA, ⁴Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA, ⁵School of Public Health, ⁶Department of Biochemistry, Indian Institute of Science, Bangalore, India, ⁷Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA; ⁸Department of Zoology, Faculty of Science, Mansoura University, Egypt

TABLE OF CONTENTS

1. Abstract

2. Introduction

3. Materials and Methods

3.1. Antibody production and characterization

3.2. Immunohistochemistry

3.3. 3H Thymidine uptake assay

3.4. 3H Riboflavin uptake assay

3.5. Immunoprecipitation

3.6. Western blot

3.7. siRNA-mediated depletion of RCP

4. Results

4.1. Immunohistochemical localization of RCP

4.2. 3H Thymidine uptake studies

4.3. 3H Riboflavin uptake studies

4.4. Western blot studies

4.5. siRNA studies

5. Discussion

6. Summary and perspective

7. Acknowledgements

8. References

1. ABSTRACT