[Frontiers in Bioscience 14, 4376-4385, January 1, 2009]
Protein microarrays: potentials and limitations
Thomas Joos1, Jutta Bachmann2
1NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr, 55, 72770 Reutlingen, Germany, 2Nokkefaret 12, 1450 Nesoddtangen, Norway
TABLE OF CONTENTS
Protein microarray technology has made enormous progress in recent years. It has been successfully applied for the identification, quantification and functional analysis of proteins in basic and applied proteome research. Protein microarrays have the potential to replace singleplex analysis systems. A variety of different analytical platforms have been developed that are likely to evolve into key technologies for the characterisation of complex samples. However, in contrast to well-established DNA microarrays, the robustness and automation needs to be demonstrated before protein microarray technology will reliably be integrated into high-throughput and routine applications. In this review we will summarise the current stage of protein microarray technology. Recent applications used for the simultaneous determination of a variety of parameters from a minute amount of sample will be described and future challenges of this cutting-edge technology will be discussed.