[Frontiers in Bioscience 15, 826-839, June 1, 2010]

[Frontiers in Bioscience 15, 826-839, June 1, 2010]

Identification of gibberellin acid-responsive proteins in rice leaf sheath using proteomics

Jiayu Gu, Ye Wang, Xu Zhang, Shihua Zhang, Yin Gao, Chengcai An

The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, P. R. China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods: 3.1. Growing conditions for rice cultivars; 3.2. Molecular cloning of OsGA20ox2 gene; 3.3. Preparation of rice leaf sheath for microscopy; 3.4. Extracting rice leaf sheath proteins; 3.5. Two-dimensional gel electrophoresis of rice leaf sheath proteins; 3.6. Image analysis of rice leaf sheath proteins; 3.7. In-gel digestion and MALDI-TOF-MS analysis of rice leaf sheath proteins
4. Results: 4.1. Semidwarf phenotype of the rice GA-deficient cultivar, Aijiaonante; 4.2. Genetic analysis of Aijiaonante; 4.3. Differential expression of rice leaf sheath proteins in Aijiaonante and Nante
5. Discussion: 5.1. The genetic basis for semidwarf characteristics of Aijiaonante; 5.2. GA-responsive proteins in rice leaf sheath
6. Acknowledgements
7. References

1. ABSTRACT

The phytohormone gibberellin acid (GA) controls many aspects of plant development. In this study, we identified proteins that are differentially expressed between the rice (Oryza sativa L.) GA-deficient cultivar, Aijiaonante, and its parental line, Nante. Proteins were extracted from rice leaf sheath and examined by 2DGE. Among more than 1200 protein spots reproducibly detected on each gel, 29 were found to be highly up-regulated by GAs in Nante, and 6 were down-regulated by GAs in Aijiaonante. These 35 proteins were identified by MALDI-TOF MS and were classified into three groups based on their putative function in metabolism, stress/defense processes and signal transduction. These data suggest that metabolic pathways are the main target of regulation by GAs during rice development. Our results provide new information about the involvement of GAs in rice development.