[Frontiers in Bioscience 15, 901-912, June 1, 2010]

Focal adhesion kinase and p53 signal transduction pathways in cancer

Vita M. Golubovskaya1, William Cance1

1Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. FAK gene structure
4. FAK ptotein structure
4.1. FAK N-terminal domain
4.2. FAK kinase domain
4.3. FAK C-terminal domain
5. Functions of FAK in cancer 5.1. Survival function
5.2. Motility function
5.3. Metastasis, invasion and angiogenesis functions
6. FAK and p53 signaling
6.1. Structure and function of p53 Protein
6.2. Mutations of p53 in cancer
6.3. p53 binds and represses FAK promoter
6.4. Direct FAK and p53 protein binding
6.5. Direct FAK and Mdm-2 protein binding
6.6. Feedback model of FAK-p53 protein interaction
7. FAK inhibitors
8. Targeting FAK protein interactions
9. Perspective
10. Acknowledgements
11. References

1. ABSTRACT

Human cancer is characterized by a process of tumor cell motility, invasion, and metastasis. One of the critical tyrosine kinases that is linked to these processes of tumor invasion and survival is the Focal Adhesion Kinase (FAK). Our laboratory was the first to isolate FAK from human tumors, and we had demonstrated that FAK mRNA was up-regulated in invasive and metastatic human breast and colon cancer samples. We have cloned FAK promoter and have found that FAK promoter contains p53 binding sites, and that p53 inhibits FAK transcription and regulates its expression in tumor samples. In addition, we have found a high correlation between FAK overexpression and p53 mutations in 600 population-based series of breast cancer patients. found that N-myc binds FAK promoter and induces FAK transcription in neuroblastoma cells. Thus, this review will be focused on FAK and p53 signal transduction pathways in cancer.