[Frontiers in Bioscience E2, 449-455, January 1, 2010]

Proteomic analysis of Alternaria alternata (Fr.) Keissler responds to COS fumigation

Tao Liu1, Li Li1, Haixia Qu2, Guoping Zhan1, Bo Liu1, Yuejin Wang1

1Chinese Academy of Inspection and Quarantine, NO.241, Huixinxijie, Chaoyang district, Beijing, 100029, P.R. China, 2Sichuan Agriculture University, NO.46, Xinkang street, Yucheng district, Yaan, 625014, P.R. China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Preparation of inoculum of A. alternate
3.2. Fumigation of A. alternata
3.3. 2-DE
3.4. Protein identification
3.5. RT-PCR for transcription analysis
4. Results
4.1. Inhibition of COS fumigation on the growth of A.alternata
4.2. The differential proteomic profiles of A.alternata by COS treatment
4.3. Transcriptional analysis of COS regulated proteins
5. Discussion
6. Acknowledgments
7. References

1. ABSTRACT

Carbonyl sulfide (COS) is a new fumigant which has been a potential alternative to methyl bromide and phosphine in many applications. In this study, we investigated the fungitoxicity of COS towards the pathogen of pear black spot disease Alternaria alternata (Fr.) Keissler (A. alternata). Moreover, proteomic analysis and RT-PCR was performed and our results showed that during the fumigation, the regulation of 21 proteins in protein expression and mRNA accumulation levels is involved, which respond to growth inhibition caused by COS. These results provide new clues for the mechanism of the fungitoxicity of COS.