Yunlong Du¹, Meiqiang Cui¹, Dan Qian¹, Lei Zhu³, Chunhong Wei¹, Ming Yuan³, Zhongkai Zhang⁴, Yi Li^{1, 2}

1Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, The National Plant Gene Research Center, Peking University, Beijing 100871, China, ²National Center for Plant Gene Research, Beijing 100101, China, ³State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China, ⁴Yunnan Provincial Key Laboratory of Agri-Biotechnology, Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan, China

TABLE OF CONTENTS

1. Abstract

2. Introduction

3. Materials and methods

3.1. Plant growth, mutant screening and plant transformation

3.2. Cloning of AtTFC B coding sequence

3.3. Quantitative real-time PCR

3.4. Examination of ovules

3.5. Leaf morphology and structure

3.6. Microtubule analyses

3.7. Flow cytometry

3.8. Data analysis and image process

4. Results

4.1. The homozygous attfc b (-/-) allele caused embryonic lethality

4.2. Plants carrying a heterozygous attfc b (+/-) allele caused enlarged cell size

4.3. Overexpression of AtTFC B restored the attfc b (+/-) phenotype and inhibited growth in wild type background

4.4. Mutation in AtTFC B altered ploidy levels and the mRNA levels of Cyclin B1;1 and Cdc2A

4.5. Microtubule arrays were affected in attfc b (+/-) mutant

5. Discussion

6. Acknowledgements

7. References

1. ABSTRACT