[Frontiers in Bioscience E2, 940-947, June 1, 2010]

Protective effects of N-acetylcysteine on homocysteine induced injury in chick embryos

Zhong-Ji Han1,2, Yi Cui1,2, Ge Song1,2, Hong-Fei Xia2, Xu Ma1,2

1Graduate School of Peking Union Medical College, Beijing, China, 2Department of Genetics, National Research Institute for Family Planning, Beijing, China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Cell culture and treatment
3.2. Embryo treatments
3.3. Embryo viability 3.4. Whole-mount terminal deoxynucleotidyl transferase-mediated biotinylated dUTP nick end labeling (TUNEL) 3.5. Extra-embryonic vascular development
3.6. ROS detection
3.7. Data analysis
4. Results
4.1. Embryo viability
4.2. Whole-mount TUNEL
4.3. Extra-embryonic vascular development
4.4. ROS detection
5. Discussion
6. Acknowledgments
7. References

1. ABSTRACT

Protective effects of N-acetylcysteine (NAC) on homocysteine (Hcy)-induced injury have been reported in vitro. However, it is not known whether NAC has a similar effect in Hcy-induced injury during embryonic development. In this study, we tested the hypothesis that exogenous NAC can attenuate Hcy-induced injury in chick embryos. Hcy-induced apoptosis and reduced embryo viability were effectively attenuated by application of exogenous NAC. NAC could also rescue Hcy-induced inhibition of extra-embryonic vascular development. 2',7'-Dichlorofluorescein diacetate, an indicator of reactive oxygen species, was detected in H9C2 cells after treatment with Hcy. The results of this study provide the first evidence that NAC can protect against the adverse effect of Hcy during chick embryo development, and suggest that these effects are at least partly mediated by oxidative stress.