[Frontiers in Bioscience E2, 1385-1393, June 1, 2010]
RBC membrane damage and decreased band 3 phospho-tyrosine phosphatase activity are markers of COPD progression
Yessica Dorin Torres-Ramos1, Alberto Martin Guzman-Grenfell1, Araceli Montoya-Estrada1, Alejandra Ramirez-Venegas2, Raul Sansores Martinez2, Fernando Flores-Trujillo2, Leticia Ochoa-Cautino2, Juan Jose Hicks1
1Departamento de Bioquimica y Biologia Molecular del Instituto Nacional de Perinatologia, Isidro Espinosa de los Reyes, Mexico, 2Departamento de Investigacion en Tabaquismo y EPOC, Instituto Nacional de Enfermedades Respiratorias (INER) "Ismael Cosio Villegas", Mexico
TABLE OF CONTENTS
Injury to red blood cell (RBC) membrane by oxidative stress is of clinical importance in chronic obstructive pulmonary disease (COPD) which leads to oxidative stress (OE) during disease progression. Here, we studied the impact of this stress on injury to RBC membrane. Blood samples from both healthy volunteers (HV, n = 11) and controlled COPD patients (n=43) were divided according to their GOLD disease stage (I=7, II=21, III=10, IV=5). Plasma levels of paraoxonase (PON) activity, protein carbonyls (PC), conjugate dienes, lipohydroperoxides (LPH) and malondialdehyde (MDA) were determined and the PTPase, and the oxidative parameters were measured in RBC ghosts. Plasma from patients with COPD showed an increased oxidation of lipids and proteins, that correlated with the disease progression. PON activity decreased from GOLD stages II to IV and correlated with an increase in LPH (p < 0.0001, r = -0.8115). There was evidence of an increase in the oxidative biomarkers in RBCs, while the PTPase activity was diminished in stage III and IV of COPD. In conclusion, OE-induced injury associated with COPD is associated with an oxidative damage to the RBC membrane, with a concomitant decrease in the PTPase activity and altered function of anionic exchanger (AE1).