[Frontiers in Bioscience E2, 351-360, January 1, 2010]

[Frontiers in Bioscience E2, 351-360, January 1, 2010]

Effects of TGF beta-1 on mouse embryo implantation and expression of H2-D1 and H2-DM

Yan-Shuai Zhang^1,2, Gao-Feng Xiong^1,2, Jing-Pian Peng¹

¹State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China, ²Graduate University of the Chinese Academy of Sciences, Beijing, 100080, China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Methods and Materials: 3.1. Animals and tissue collection; 3.2. Real-time PCR; 3.3. Western blotting; 3.4. Immunohistochemistry; 3.5. Statistical analysis
4. Results: 4.1. Effect of anti-TGF-beta1 antibody treatment on embryo implantation 4.2. Effect of anti-TGF-beta1 antibody treatment on H2-D1; 4.3. Effect of anti-TGF-beta1 antibody treatment on H2-DM; 4.4. Effect of exogenous hrTGF-beta1 treatment on embryo implantation; 4.5. Effect of exogenous hrTGF-beta1 treatment on H2-D1; 4.6. Effect of exogenous hrTGF-beta1 treatment on H2-DM
5. Discussion
6. Acknowledgement
7. Reference

1. ABSTRACT

We investigated the mechanism that TGF-beta1 influences the immuno-environment at maternal-fetal interface and affects embryo implantation, using mouse uterine horn injection model. The expression of MHC I antigen (H2-D1) and the chaperone of MHC II antigen (H2-DM) after anti-TGF-beta1 antibody or hrTGF-beta1 treated pregnant mice were examined by real-time PCR, western blotting and immunohistochemistry. The results showed that the number of implanted embryos of anti-TGF-beta1 antibody-treated mice was decreased compared with the control. The expression of H2-D1 and H2-DM on days 6 and 7 treated uteri was increased both at mRNA and protein levels. In hrTGF-beta1 treated group, the expression of H2-D1 and H2-DM protein was decreased, and the number of implanted embryos was slightly increased. Immunohistochemical studies revealed that H2-D1 and H2-DM were mainly localized to the primary decidual zone. The anti-TGF-beta1 antibody and exogenous hrTGF-beta1 treatment altered the intensity of H2-D1 and H2-DM signal but did not change their localization. These observations suggested that injection of anti-TGF-beta1 antibody affected the number of mouse embryo implantation, and regulated the expression of H2-DM and H2-Q10.