[Frontiers in Bioscience E3, 227-232, January 1, 2011]

Relationship between RANTES and dendritic cells in ovarian cancer patients

Iwona Wertel 1, Rafal Tarkowski1, Wieslawa Bednarek1, Jan Kotarski 1

11st Department of Gynaecology, University School of Medicine, Lublin, Poland

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Patients
3.2. Methods
3.3. Cell preparation
3.4. Flow cytometric analysis
3.5. Statistical analysis
4. Results
4.1. Concentration of RANTES in the peritoneal fluid and plasma of women with ovarian cancer and serous cystadenoma
4.2. Concentration of RANTES in patients with different stage, grade and histologic type of ovarian cancer
4.3. Concentration of RANTES in the plasma of women with ovarian cancer or serous cystadenoma and in the control group
4.4. The percentage of DCs in PF and PB of women with ovarian cancer
4.5. The percentage of DCs in PF and PB of women with serous cystadenoma
4.6. The percentage of DCs in PF and PB of women with ovarian cancer and benign tumors
4.7. Correlation between concentration of RANTES and dendritic cell subsets
4.8. Correlation between serum Ca-125 and RANTES concentrations
5. Discussion
6. Acknowledgment
8. References

1. ABSTRACT

This study was undertaken to evaluate RANTES levels in the peritoneal fluid (PF) and plasma of patients with ovarian cancer (n=73), serous cystadenoma (n=32) or normal controls (n=9). RANTES levels were correlated to myeloid and lymphoid dendritic cells (DCs). RANTES levels were evaluated using the ELISA assay. DCs were quantified using flow cytometry. The PF and plasma RANTES concentrations were elevated in the ovarian cancer (OVC) patients when compared to the patients with benign tumor (the reference group). Plasma levels of RANTES were higher in OVC patients compared with the reference group and with the controls. There were no significant differences in the plasma RANTES levels based on tumor stage, grade or histology. Women with serous cystadenocarcinoma, clear cell carcinoma and endometrioid cystadenocarcinoma had significantly higher PF RANTES levels than patients with undifferentiated carcinoma. Women with clear cell carcinoma and patients with endometrioid cystadenocarcinoma had higher PF RANTES levels than women with mucinous cystadenocarcinoma. We concluded that RANTES production in the peritoneal cavities of OVC patients depends on the histological type of the tumor cells.