[Frontiers in Bioscience 17, 892-908, January 1, 2012]

The biology of equine mesenchymal stem cells: phenotypic characterization, cell surface markers and multilineage differentiation

Jasmine Penny1, Pat Harris2, Kevin Shakesheff3, Ali Mobasheri1

1Musculoskeletal Research Group, Division of Veterinary Medicine, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD, United Kingdom,2Equine Studies Group, WALTHAM Centre for Pet Nutrition, Waltham-on-the-Wolds, Leicestershire, LE14 4RT, United Kingdom,3 Wolfson Centre for Stem cells, Tissue Engineering and Modelling (STEM), Centre for Biomolecular Sciences, University of Nottingham, University Park Campus, Nottingham, NG7 2RD, United Kingdom

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Proliferation of equine MSC research
4. Equine bone marrow derived MSCs
4.1. Characterization of bone marrow derived MSCs
4.2. Factors affecting bone marrow derived MSC differentiation
4.2.1. BMPs and their effects on bone marrow derived MSCs
5. Equine adipose tissue derived MSCs
5.1. Characterization of adipose tissue derived MSCs
5.2. Comparison of adipose tissue derived MSCs with bone marrow derived MSCs
6. Equine umbilical cord blood and matrix derived MSCs
7. Equine peripheral blood derived MSCs
8. Summary and conclusions
9. Acknowledgements
10. References

1. ABSTRACT

Mesenchymal stem cells (MSCs) are multipotent stem cells that can give rise to a range of connective tissue cells including osteoblasts, chondrocytes and adipocytes. MSCs have been isolated from humans and a variety of animal species including rodents, dogs, horses and rabbits. There is currently no consensus on how these cells are identified and characterized. This is partly due to the lack of standardized specific cell surface markers for MSCs. The aim of this review is to examine the literature on equine MSCs and establish whether there is a well-defined phenotype for these cells. Equine MSCs have been obtained from four main sources, bone marrow, adipose tissue, umbilical cord (blood and matrix) and peripheral blood. MSCs from these tissue sources have been shown to undergo chondrogenic, adipogenic and osteogenic differentiation. However the markers used to identify these cells vary significantly in the literature. Despite this, CD90 and CD34 seem to be reliable positive and negative markers respectively. Our understanding of the biology of equine MSCs will benefit from better reagents for their phenotypic characterization. The antibodies and molecular probes needed for the reliable identification of equine MSCs are not standardized and this is a high priority for future research.