[Frontiers in Bioscience E4, 101-110, January 1, 2012]

Bioconjugated nanomaterials on devices for infectious disease diagnostics

Concepcion Ponce1, Philip Ian Padilla2, 3

1Department of Chemistry, College of Arts and Sciences, University of the Philippines, Visayas, Miag-ao, Iloilo, Philippines, 2Division of Biological Sciences, College of Arts and Sciences, and the National Institute of Molecular Biology and Biotechnology, University of the Philippines, Visayas, Miag-ao, Iloilo, Philippines; 3Dept of Molecular Epidemiology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Biosensors
3.1. Functionalization and bioconjugation of nanomaterial probes
3.2. Top down and bottom up nanomanipulation of transducer surfaces
3.2.1. Nanoimprint lithography
3.2.2. Nanosphere lithography
3.2.3. Dip pen lithography
3.2.4. Oblique angle deposition and related methods
4. Summary points
5. Acknowledgements
6. References

1. ABSTRACT

The successful use of the nanoscale-dependent properties of nanomaterials on infectious diseases diagnostics devices lies on a well-engineered surface of both the probes and the transducer. Engineering probe and transducer surfaces involve functionalization of nanomaterials, conjugation to biorecognition elements and nanopatterning. This review presents the most common and most promising functional groups, bioconjugation schemes and patterning strategies of nanomaterials on sensing devices and their specific application to infectious pathogen detection.