[Frontiers in Bioscience E4, 1071-1080, January 1, 2012]

Prostaglandin E synthase is regulated in postnatal mouse testis

Yanjun Zhang1, Yi Du, Dengyu Wang1, Xuekun Li1, Renwei Jing1, Weihua Kong1

1Institute of Developmental Biology, School of Life Sciences, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, Shandong University, Jinan 250100, P.R. China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Animals
3.2. Tissue preparation
3.3. Isolation and culture of Sertoli cells
3.4. Isolation of spermatocytes and round spermatids
3.5. Western blot analysis
3.6. Immunohistochemistry
3.7. Immunofluorescent staining of isolated Sertoli cells and germ cells
3.8. Statistical analysis
4. Results
4.1. Expression level of cPGES in mouse testis during postnatal development
4.2. Distribution of cPGES in mouse seminiferous epithelium during postnatal development by immunohistochemistry
4.3. Immunofluorescent localization and expression level of cPGES in the isolated Sertoli cells and germ cells
4.4. Expression of cPGES in mouse male genital organs
5. Discussion
6. Acknowledgement
7. References

1. ABSTRACT

Prostaglandins have important roles in the male reproductive system. In this study, we report on the distribution and regulation of cPGES during postnatal development of mouse testis. The expression of cPGES was weak in testis 5 days after birth and increased through the 10th and 15th day. From the 20th day onward, the cPGES expression in testis reached the level of adult mice. cPGES was expressed at a constantly low level in Sertoli cells in the testis from infant to adult stages. With the occurrence of meiosis during puberty, a high level of cPGES was detected in the spermatocytes and round spermatids, which was then maintained throughout the adulthood. In addition, cPGES was found highly expressed in the epididymis, seminal vesicles and vas deferens This suggests that cPGES in Sertoli cells in infant to juvenile mouse testis contributes to a basic PGE2 synthesis in seminiferous tubules. However, the high level of cPGES in spermatocytes and spermatids may maintain a high amount of PGE2 in seminiferous tubules, which may be tightly coupled with the spermatogenic cycle.