[Frontiers in Bioscience E4, 1802-1812, January 1, 2012]

Prognostic relevance of the expressions of CAV1 and TES genes on 7q31 in melanoma

Laura, Vizkeleti1, Szilvia, Ecsedi1,2, Zsuzsa, Rakosy1,2, Agnes, Begany3, Gabriella, Emri3, Reka, Toth1,2, Adrienn, Orosz4, Attila, Gabor, Szollosi5, Gabor, Mehes6, Roza, Adany1,2, Margit, Balazs1,2

1Department of Preventive Medicine, Faculty of Public Health, Medical and Health Science Center, University of Debrecen, Hungary 4028, 2 Public Health Research Group of the Hungarian Academy of Sciences, University of Debrecen, Hungary 4028, 3Department of Dermatology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Hungary 4028, 4Clinical Research Center, Medical and Health Science Center, University of Debrecen, Hungary 4028, 5Department of Physiology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Hungary 4028, 6Department of Pathology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Hungary 4028

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Melanoma tissue samples
3.2. Cell lines
3.3. Fluorescence in situ hybridization and scoring of the signals
3.4. RNA extraction and QRT-PCR
3.5. Tissue microarrays and immunostaining
3.6. Statistical analysis
4. Results
4.1. Chromosome 7 and 7q31 copy number alterations in primary melanomas
4.2. Correlation of 7q31 alterations with clinical-pathological parameters of patients
4.3. Alterations of 7q31 in primary melanomas and their corresponding metastasis pairs
4.4. CAV1 and TES mRNA and protein expression in primary melanomas
4.5. Copy number alterations, mRNA and protein expression levels of the CAV1 and TES genes in primary melanomas and melanoma cell lines
5. Discussion
6. Acknowledgement
7. References

1. ABSTRACT

The 7q31 locus contains several genes affected in cancer progression. Although evidences exist regarding its impact on tumorigenesis, the role of genetic alterations and the expressions of locus-related genes are still controversial. Our study aimed to define the 7q31 copy number alterations in primary melanomas, primary-metastatic tumor pairs and cell lines. Data were correlated with clinical-pathological parameters. Genetic data show that 7q31 copy number distribution was heterogeneous in both primary and metastatic tumors. Extra copies were highly accompanied by chromosome 7 polisomy, and significantly increased in primary lesions with poor prognosis. Additionally, we determined the mRNA and protein levels of the locus-related CAV1 and TES genes. TES mRNA level was associated with metastatic location. CAV1 mRNA and protein levels were significantly higher in thicker tumors, however, lack of protein was also observed in a subpopulation of thin lesions. Expressions of CAV1 and TES were not associated with 7q31 alterations. In conclusion, 7q31 amplification can predict unfavorable outcome. Alterations of TES mRNA level may predict the location of metastasis. CAV1 possibly affect the cancer cell invasion.