[Frontiers in Bioscience, Elite, 7, 417-422, June 1, 2015]

Physical exercise reduces synthesis of ADMA, SDMA, and L-Arg

Graziano Riccioni 1 , Luca Scotti 2 , Maria Teresa Guagnano 3 , Gabriella Bosco 3 , Valentina Bucciarelli 2 , Emanuela Di Ilio 2 , Lorenza Speranza 3 , Filippo Martini 2 , Tonino Bucciarelli 2

1Cardiology Care Unit, San Camillo de Lellis Hospital, Manfredonia, Foggia, Italy, 2Clinical Biochemistry, Department of Biomedical Science, G. D’Annunzio, University of Chieti, Italy, 3Department of Internal Medicine and Aging Science, University G. D’Annunzio, Chieti, Italy


1. Abstract
2. Introduction
    2.1. Study design
    2.2. Patients
    2.3. Sample collection, storage and preparation
    2.4. Biochemical analysis
    2.5. Physical exercise
    2.6. Statistical analysis
3. Results
    3.1. Characteristic of subjects
4. Discussion
5. References


Increased levels of asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and low plasma level of L-arginine (L-ARG) are all conditions likely to decrease nitric oxide (NO) production. Aim of this study is to evaluate ADMA, SDMA, and L-ARG plasmatic levels before and after physical exercise in patients with coronary artery disease (CAD). We studied 30 patient with mean age 52 ± 4.5 years. After inclusion in the study, before the execution of physical exercise, heparinized blood sample was drawn from an indwelling arterial line for determination of ADMA, L-ARG and SDMA (baseline values). Subsequently a blood sample was drawn after the physical exercise. The mean plasma concentrations of ADMA (0.68 0.06 vs 0.48 ± 0.05 mol/L) and SDMA (0.45 ± 0.03 vs 0.30 ± 0.03 ýmol/L) were significantly lower after physical exercise in comparison to baseline value, while L-ARG mean levels were increased (44.20 ± 10.5 vs 74.13 ± 11.2 ýmol/L). Physical exercise has a beneficial effect by reducing plasmatic ADMA and SDMA levels, and increasing L-ARG substrate for endothelial NO.


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Key Words: ADMA, SDMA L-arginine, Nitric Oxide; Coronary Artery Disease, Physical Exercise

Send correspondence to: Graziano Riccioni, Intensive Cardiology Care Unit, San Camillo de Lellis Hospital, 71043 Manfredonia, Foggia, Italy, Tel: 39-0884-510470, Fax: 39-0882-227022, E-mail: griccionI@hotmail.com