[Frontiers in Bioscience, Elite, 9, 33-43, January 1, 2017]

Novel biogenic synthesis of silver nanoparticles and their therapeutic potential

Navdeep Raghuwanshi 1 , Ashish Pathak 2 , Alok Patel 1 , Priya Vashisth 1 , Harmeet Singh 1 , Amit Kumar Srivastava 2 , Vikas Pruthi 1

1Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India, 2Sapience Bioanalytical Research Lab, Bhopal 462021, Madhya Pradesh, India

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
    3.1. Chemicals
    3.2. Plant material
    3.3. Extract preparation
    3.4. Biogenic synthesis of PaAgNPs
    3.5. Phytochemical screening
    3.6. Characterization of PaAgNPs
      3.6.1. Spectroscopic analysis
      3.6.2. Microscopic analysis
      3.6.3. XRD analysis
      3.6.4. Hydrodynamic size (DLS) and zeta potential analysis
    3.7. Antioxidant activity
      3.7.1. Antiradical (DPPH) assay
    3.8. Anti-inflammatory activity
      3.8.1. Experimental animals
      3.8.2. Acute oral toxicity studies
      3.8.3. Carrageenan-induced rat paw edema
      3.8.4. Statistical analysis
4. Results and discussion
5. Conclusion
6. Declaration of conflicting interests
7. Acknowledgements
8. References

1. ABSTRACT

Here, we explored the medicinal uses of the novel biogenic silver nanoparticles of Pterospermum acerifolium (PaAgNPs) as a cost effective, eco-friendly, reducing and stabilizing compounds. The formation of PaAgNPs was confirmed by changing its color from colorless to yellowish brown, with maximum absorbance at 417nm. FTIR spectrum of PaAgNPs suggested the presence of polycyclic compound similar to betulinic acid which plays as a capping agent and provided stability to PaAgNPs. FESEM and HRTEM images depicted the spherical shape of synthesized biogenic silver nanoparticles with an average particle size range of 10-20nm. The EDX spectrum of the solution confirmed the presence of elemental silver signals. The crystalline nature of PaAgNPs was identified by XRD technique and its stability was recorded using Zeta potential analyzer. The antioxidant potential was assayed using diphenyl-β-picrylhydrazyl (DPPH). Maximum free radical scavenging action of PaAgNPs was 69.52% as compared to 63.53% for PALE. Using a model of carrageenan-induced paw edema in rats, PaAgNPs showed two-fold enhanced anti-inflammatory activity in vivo.

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Key Words: PALE, PaAgNPs, Carrageenan, Paw Edema, DPPH

Send correspondence to: Vikas Pruthi, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT-R), Roorkee-247667, India, Tel: 091-1332-285530, Fax: 091-1332-273560, E-mail: vikasfbs@gmail.com