[Frontiers In Bioscience, Scholar, 9, 375-394, June 1, 2017]

Multi-omics and male infertility: status, integration and future prospects

Ashima Sinha1, Virendra Singh2, Savita Yadav1

1All India Institute of Medical Sciences (AIIMS), Department of Biophysics, New Delhi, India,2 Jawaharlal Nehru University (JNU), School of Life Sciences, New Delhi, India

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Epigenomics
3.1. Outline of epigenetic mechanisms and their regulation
3.2. Epigenetic transformations in sperm chromatin during spermatogenesis
3.3. DNA methylation and male infertility
3.4. Global DNA methylation status in Oligozoospermic and Asthenozoospermic (AS) infertile males
4. Genomics and male infertility
5. Proteomics and male infertility
6. Metabolomics and male infertility
7. Integrative links among multi-omics approaches for male infertility
8. Future of integration of multi-omics data in male infertility
9. Acknowledgments
10. References

1. ABSTRACT

Within the cell, gene expression analysis is the key to gain information about different cellular and physiological events. The multifaceted route of fertilization is a combination of different processes, which include production, maturation and ejaculation of the sperm, its travel through the female genital tract, followed by the ultimate fusion of the fertile sperm with the egg. Early embryogenesis and gametogenesis as well as gene expression at tissue level and global gene silencing are under different levels of stringent epigenetic checks. Moreover, transcriptome (expressed segment of the genome in form of RNA) and the proteome (expressed set of genomic proteins) contribute uniformly to the overall cellular gene expression. In both normal and pathophysiological environments, this gene expression is altered across various levels viz., genome variations, post-transcriptional modifications, protein expression and post translational modifications. Consequently, more informative conclusions can be drawn through a new ‘omics’ approach of system biology, which takes into account all the genomics, epigenomics, proteomics, and metabolomics findings under one roof, thus computing the alterations in all the entities (genes, proteins, metabolites) concurrently.

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Key Words: Male Infertility, Integrome analysis, Multi-omics, Proteomics, Genomics, Metabolomics, Review

Send correspondence to: Savita Yadav, All India Institute of Medical Sciences (AIIMS), Department of Biophysics, New Delhi, India, Tel: 112-654-6445, Fax: 112-658-8641, E-mail: savita11@gmail.com