[Frontiers in Bioscience, Landmark, 24, 628-647, March 1, 2019]

One-step strategy for chondral defect repair

Ying Zhang 1 , Hongbo Tan 2 , Guangxing Chen 1 , Weinan Zeng 1 , Junli Liu 1 , Liu Yang 1 , Fuyou Wang 1

1Center for Joint Surgery, Southwest Hospital, Third Military Medical University, No. 29 Gaotanyan Street, Shapingba District, Chongqing 400038, P.R. China, 2Department of Orthopaedics, Kunming General Hospital, No. 212 Daguan Road, Xishan District, Kunming, Yunnan 650228, P.R. China


1. Abstract
2. Introduction
3. Materials and methods
    3.1. Animals
    3.2. Col-II isolation and purification
    3.3. Sol-gel transition of Col-II
    3.4. BMDC isolation and cell-sol mixture preparation
    3.5. Chondral defect creation and gel implantation
    3.6. MRI and macroscopic, and histological evaluation
    3.7. Cell imaging
    3.8. Statistical analysis
4. Results
    4.1. MRI observation
    4.2. Macroscopic and microscopic observation
    4.3. Histological results
    4.4. Cell imaging
5. Discussion
6. Acknowledgements
7. References


One-step clinical therapies of articular cartilage defects remains a challenge. In this study, a strategy was proposed to utilize type II collagen (Col-II) gels with autologous bone marrow-derived cells (BMDCs) embedded to repair full-thickness chondral defects. Its feasibility and efficacy were further assessed in a minipig model. An 8-mm full-thickness chondral defect was created on the femoral trochlea of two knees in Guizhou minipigs. One knee received Col-II gels with BMDCs implantation versus the untreated one as control. After 1, 3, and 6 months operation, the animals were sacrificed, and the repair outcomes of chondral defects were evaluated using Magnetic Resonance Imaging (MRI), macro- and microscopic observation, and histological analysis. The treatment group showed significantly better repair outcomes of chondral defects than that in the control group at each time point (P < 0.01). Furthermore, the image showed that the repaired tissue in the treatment group was more similar to the surrounding healthy cartilage tissue. Based on the hyaline-like tissue regenerated ability, this one-step strategy provides a promising therapeutic potential for clinical application.


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Abbreviations: MSCs: senchymal stem cells; Col-II: type II collagen; MRI: Magnetic Resonance Imaging; ACI: autologous chondrocyte implantation; MOCART: Magnetic resonance Observation of CArtilage Repair Tissue; ICRS: International Cartilage Repair Society; EDTA: Ethylene diamine tetraacetic acid; FISH: Fluorescence in situ hybridization; IHC: Immunohistochemistry

Key Words: Articular Cartilage Defect, BMDCs, Type II Collagen Gel, Regeneration, Repair

Send correspondence to: Fuyou Wang, Center for Joint Surgery, Southwest Hospital, Third Military Medical University, No. 29 Gaotanyan Street, Shapingba District, Chongqing 400038, P.R. China, Tel: 86-68765290, Fax: 86-68765293, E-mail: wfy731023@163.com