[Frontiers In Bioscience, Landmark, 24, 961-970, March 1, 2019]

The antitumor effect of resveratrol on nasopharyngeal carcinoma cells

Huisheng Xiong1, Jun Cheng2, Shen Jiang1, Jun Wen1,Yang Jian3, Lu Wei3, Zhang Zhe3, Jiang Fu-qiang3, Xie Peng3

1Department of Traditional Chinese Medicine, Chongqing Cancer Institute and Hospital and Cancer Center, Chongqing 400030, P. R. China, 2Department of Oncology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400030, P. R. China, 3Department of Interventional Medicine, Chinese PLA Navy General Hospital, 6 Fucheng Road, Beijing, People’s Republic of China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Cell culture and treatment
3.2. Cell proliferation assay
3.3. Western blot assay
3.4. Cell apoptosis assay
3.5. Transfection
3.6. Statistical analysis
4. Results
4.1. Resveratrol inhibits proliferation of NPC cells
4.2. Resveratrol induces apoptosis of NPC cells
4.3. Resveratrol suppresses the expression of Survivin
4.4. Overexpressed Survivin counteracts the effects of resveratrol in NPC cells
5. Discussion
6. Acknowledgment
7. References

1. ABSTRACT

The anti-tumor effect of resveratrol has been observed in many cancers. Here, we examined the anti-tumor activity of resveratrol in human nasopharyngeal carcinoma (NPC) cells. Resveratrol, in a dose-dependent manner, inhibited proliferation related proteins (Ki67, PCNA), and cell proliferation, and reduced apoptosis related proteins (cleaved caspase-3, cleaved caspase-9) and apoptosis in nasopharyngeal carcinoma cells. Resveratrol treatment inhibited the increased-expression of Survivin in NPC cells, while the overexpressed Survivin counteracted the effect of resveratrol on cell proliferation and apoptosis in NPC cells, thus establishing Resveratrol-induced reduction in increased-survivin in NPC cells as the underlying mechanism. These findings show that resveratrol can be used to modify the cell growth and death in NPC cells.

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Abbreviations: NPC, nasopharyngeal carcinoma; EBV, Epstein-Barr virus; CCK-8, Cell Counting Kit-8; SD, standard deviation.

Key Words: Resveratrol, Antitumor, Nasopharyngeal Carcinoma, Survivin

Send correspondence to: Huisheng Xiong, Department of Traditional Chinese Medicine, Chongqing Cancer Institute & Hospital & Cancer Center, 81 Hanyu Road, Shapingba District, Chongqing 400030, P. R. China. Tel: 15823190731; FAX:023-65311341,E-mail: xionghuishengcq@163.com.