[Frontiers in Bioscience, Elite, 12, 126-138, Jan 1, 2020]

Amplification of speckle-microscope signal by using gold nanoparticle stable of contents

Onega V. Ulianova1,2, Nadejda N. Filonova1, Sergey S. Ulyanov2, Lev A. Dykman3, Olga S. Larionova4, Valentina A. Feodorova1

1Federal Research Center for Virology and Microbiology, Branch in Saratov, Saratov, 410028, Russia, 2Saratov State University, Saratov, 410012, Russia, 3Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russia, 4Saratov State Agrarian University, Saratov, 410012, Russia

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
    3.1. Detection of intensity fluctuations, scattered by moving bacterial cells.
    3.2. Set-up of speckle-microscope
4. Results
    4.1. Computer simulation of the process of speckle formation in the speckle-microscope. Influence of interference effects on the formation of speckles in speckle-microscope
    4.2. Dependence of speckle pattern structure on the position of speckles observation plane in a speckle-microscope
    4.3. Dependence of the spatial structure of speckles on the number of scatterers
    4.4. Simulation of the process of a speckle formation in the case of scattering of strongly focused laser beam on single bacteria, surrounded by a set of nanoparticles, connected to the cell via CT-Mab-Au complex
    4.5. Experimental study of speckles with a small number of scatterers forming at the scattering by the samples, containing combined “chlamydia trachomatis bacterial cell”-“monoclonal antibody”-“gold nanoparticles” system
      4.5.1. Sample preparation
      4.5.2. Scanning of samples, containing combined “Chlamydia trachomatis bacterial cell”-“monoclonal antibody”-“gold nanoparticle” system with a strongly focused laser beam
5. Discussion
6. Acknowledgments
7. References

1. ABSTRACT

Speckle-microscopy is used for the detection of Chlamydia trachomatis in aqueous suspensions or on slides. Monoclonal antibody tagged with gold nanoparticles that form biospeckles have also been used with a small number of scatterers. By devising a prototype of laser scanning speckle-microscope, we now demonstrate that the signal of speckle microscope can be significantly amplified in the presence of gold nanoparticles. This enhancement of signal emitted from gold nanoparticles offers a better approach to the detection of C. trachomatis.

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Key Words:Chlamydia trachomatis, Gold Nanoparticles, Laser, Speckle, Microscopy

Send correspondence to: Valentina A. Feodorova, Federal Research Center for Virology and Microbiology, Branch in Saratov, Saratov, 410028, Russia, Tel: 7-8452-200-825, Fax: 7-8452-200-830, E-mail: feodorov-av@mail.ru