Angiotensinogen
Complete loss of plasma immunoreactive angiotensin I
Profound hypotension
Homozygous -/-
Modest delay in glomerular maturation
Develop renal cortex lesions by week 3
Local up-regulation of PDGF-B and TGF-ß1 mRNA in renal cortex
Down-regulation of PDGF-A in renal papilla
Chronic hypertension
Increased renal renin mRNA
Database link:
| Line Name | DNA Construct | |
|---|---|---|
| 1. | RAS (+/-) | The genomic DNAs containing the angiotensinogen locus were isolated from a phage library of C57BL/6 DNA. 418 bp of the 2nd exon and a portion of the preceding intron of.... |
| 2. | Agt (+/-) | To disrupt the Agt gene, the targeting plasmid was made using an 8-kb fragment of strain 129 genomic DNA containing exons 2 and 3 of the gene. The 5' end of the fragmen.... |
| 3. | RAS (-/-) | The genomic DNAs containing the angiotensinogen locus were isolated from a phage library of C57BL/6 DNA. 418 bp of the 2nd exon and a portion of the preceding intron of.... |
| 4. | Agt (-/-) | To disrupt the Agt gene, the targeting plasmid was made using an 8-kb fragment of strain 129 genomic DNA containing exons 2 and 3 of the gene. The 5' end of the fragmen.... |
| 5. | Agt (2/1, 2/2) | Smithies and Kim (1994) (G00-641-971) used gap-repair targeting in conjunction with an O-type (insertional) targeting construct to create a complete tandem duplication o.... |
