Normal development
Normal metabolism
Development of dietary obesity, without development of insulin resistance or diabetes
Failure of expression in adipose tissue tumor necrosis factor-alpha in obese mice

Hotamisligil, R. S. Johnson. R. J. Distel, R. Ellis, V. E. Papaioannou & B. M. Spiegelman:
Uncoupling of Obesity from Insulin Resistance Through a Targeted Mutation in aP2, the Adipocyte Fatty Acid Binding Protein.
Science 274, 1377 (1996)

((ap2))

	Line Name	DNA Construct
1.	ap2 (+/-)	The Ap2 vector was derived from a 4.5-kb BamHI-SstI genomic fragment. The Ap2 gene was interrupted after the transcription start site and before a....
2.	ap2 (-/-)	The Ap2 vector was derived from a 4.5-kb BamHI-SstI genomic fragment. The Ap2 gene was interrupted after the transcription start site and before a....
3.	aP2-DT strain 12	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
4.	aP2-DT strain 13	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
5.	aP2/Ha-ras (high)	The adipose-specific Ha-ras transgene was constructed using the fat-specific promoter/enhancer from the fatty acid-binding protein gene, alpha-P2 (gift of Dr. B.M. Spieg....
6.	aP2-DT strain 2	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
7.	aP2-DT strain 6	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
8.	aP2-DT strain 18	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
9.	aP2-DT strain 1	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
10.	aP2-DT strain 8	To target expression of the DT-A gene to adipose tissue, the regulatory region from the adipocyte-specific adipocyte aP2 (aP2) gene was used. This 5.4 kb fragment was p....
11.	aP2/Ha-ras (mod)	The adipose-specific Ha-ras transgene was constructed using the fat-specific promoter/enhancer from the fatty acid-binding protein gene, alpha-P2 (gift of Dr. B.M. Spieg....
12.	AP-2 (-/-)	The targeting vector contains HSV-tk and PGK-neo cassettes flanked by AP-2 genomic DNA (5' arm, 5.1-kb EcoRI-BglII fragment containing exon 4; 3' a....