apolipoprotein B
20% reduction in plasma cholesterol levels in heterozygotes
70% reduction in plasma apolipoprotein B in heterozygotes
Protection from diet-induced hypercholesterolemia in heterozygotes
Hypobetalipoproteinemia and developmental abnormalities in homozygotes (Homanics et al., 1993)
Production of truncated apoB protein
Markedly decreased plasma concentrations of apoB, beta lipoproteins, and total cholesterol
Reduced plasma triglyceride concentrations
Fasting chylomicronemia
Reduced high density lipoprotein cholesterol
Increased incidence of exencephalus and hydrocephalus Resorption of most homozygous embryos by mid-gestation (Farese Jr. et al., 1995)Homanics et al., 1993
Farese Jr. et al., 1995
apo B
Homozygous mice
Embryonic lethality by gestational day 9 Heterozygous mice Normal triglycerides
Lower total HDL, LDL cholesterol levels
Incomplete neural tube closure
Some development of hydrocephalus
Male infertility
Reduced levels of hepatic and intestinal apo mRNA
Reduced transport rates of HDL cholesterol and apo A-1
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Huang, 1995
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apo B gene knockout in mice results in embryonic lethality in homozygotes and neural tube defects, male infertility, and reduced HDL cholesterol ester and apo A-I transport rates in heterozygotes.
J Clin Invest 96, 2152-2161 (1995) [96066679]
| Line Name | DNA Construct | |
|---|---|---|
| 1. | apoB (+/-) | An approximately 11-kb Bglll fragment containing exon 26 of the Apob gene was isolated from STO cell DNA. Two oligonucleotides which encode peptides of human beta(s)-gl.... |
| 2. | apoB (-/-) | An approximately 11-kb Bglll fragment containing exon 26 of the Apob gene was isolated from STO cell DNA. Two oligonucleotides which encode peptides of human beta(s)-gl.... |
| 3. | Apob (-/-) | A neomycin resistance gene (neo) containing the RNA polymerase II promoter and a herpes simplex virus thymidine kinase (tk) polyadenylation signal was subcloned into the.... |
| 4. | Apob (+/-) | A neomycin resistance gene (neo) containing the RNA polymerase II promoter and a herpes simplex virus thymidine kinase (tk) polyadenylation signal was subcloned into the.... |
