Apo E

 Rampant atherosclerosis

 Decreased efflux of LDL derived cholesterol from macrophages

 Increased lipid peroxidation of plasma
 Increased lipid peroxidation of LDL and VLDL
 17-ß-estradiol HRT decreases atherosclerotic lesions of both male and female ovariectomized (OVX) mice
 17-alpha-estradiol and/or Tamoxifen HRT have no effect on OVX females

Hayek et al., June 1994
Hayek et al., December 1994


METHODOLOGY ( FROM T-BASE)

((apoe))

Line Name DNA Construct
1. apoE (+/-) The apoE (apolipoprotein E) gene was cloned from a BALB/c genomic lambda phage library (Stratagene) using a mouse apoE cDNA probe; 6.8 kb of total homology was included....
2. apoE/pJPB63 (-/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
3. apoE/pNMC109 (-/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
4. apoE/pNMC109 (+/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
5. apoE/pJPB63 (+/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
6. apoE/pJPB63 (-/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
7. ApoE (+/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
8. ApoE (-/-) Mouse genomic DNA, isolated from STO cells and fully digested with EcoRI was used to make a library in lambda phage. The library was screened with an apoE-sp....
9. apoE (-/-) The apoE (apolipoprotein E) gene was cloned from a BALB/c genomic lambda phage library (Stratagene) using a mouse apoE cDNA probe; 6.8 kb of total homology was included....


Database links

OMIM MEDLINE Citations Cardiff Human Gene Mutation Database Gene Map GDB MEDLINE Neighbors