Bcl-2

Normal fertility in heterozygous mice
Gray hair
Polycystic kidney
Fulminant lymphoid apoptosis
Death 10 days to 10 weeks after birth

Nakayama et al., 1993
Veis et al., 1993

Bcl-2 alpha beta

Viable
Normal CNS
Normal intestine
Normal skin
Normal number of cells of hematopoetic lineage and T cells
Normal T cell response to Anti-CD3, Con A, phorbol 12-myristate 13-acetate plus ionomycin, IL-2, LPS, and anti-IgM antibody
Smaller in size
Small auricles
Gray hair at 4-5 weeks of age
Polycystic kidney disease
Marked decrease in lymphocytes within a few weeks after birth

Disappearance of CD8+ T cells

Disappearance of CD4+ T cells

Nakayama et al., 1994

Failure to inhibit central tolerance by bone marrow antigen expression

Reference:

Lang J, Arnold B, Hammerling G, Harris AW, Korsmeyer S, Russell D, Strasser A, Nemazee D.: Inhibition of antigen-mediated clonal elimination of peripheral B cells in an antigen dose-dependent manner and promotion of receptor editing in autoreactive, immature B cells. J Exp Med 1997 Nov 3;186 (9):1513-1522.

((bcl-2))

	Line Name	DNA Construct
1.	Bcl2 (+/-)	A genomic plasmid clone that contained a 5.6 kb EcoRI fragment encompassing the 3' end of intron II and most of exon III was digested with Pstl, and two inter....
2.	Bcl2 (-/-)	A genomic plasmid clone that contained a 5.6 kb EcoRI fragment encompassing the 3' end of intron II and most of exon III was digested with Pstl, and two inter....