Viable
Fertile
Lack of overt physical or behavioral abnormalities
Production of normal numbers of offspring from UbCKmit deficient mice
Unimpaired motility patterns of isolated spermatozoa

Steeghs, 1995

Mice deficient in ubiquitous mitochondrial creatine kinase are viable and fertile.
Biochim Biophys Acta 1230, 130-138 (1995) [95345105]

((creatine kinase))

	Line Name	DNA Construct
1.	ActCK(b)-7001	The 530 bp promoter region of the rat skeletal muscle alpha-actin promoter from pIBI20 was excised using KpnI and SphI. This fragment was cloned in....
2.	ActCK(b)-7002	The 530 bp promoter region of the rat skeletal muscle alpha-actin promoter from pIBI20 was excised using KpnI and SphI. This fragment was cloned in....
3.	HCACK(b)-4F	pHCACK(b) was derived by cloning the -480 to +70 bp HindIII to EcoRI, promoter region of the human cardiac alpha-actin promoter from pHC21 CAT into....
4.	M-CK (+/-)	The replacement vector 129-pRV8.3 was derived from a 9.0-kbp genomic BamHl fragment spanning exons 1-5 of the creatine kinase, muscle (CKM) gene, that originated from a....
5.	Prkar2b (+/-)	Details of the knockout construction have not been given.
6.	UbCKmit (-/-) ES ce	Starting from a 7-kbp SstI subclone containing the complete UbCKmit (ubiquitous mitochondrial creatine kinase) gene, a replacement-type vector for homologous....
7.	Dp71/mdx	A 1.8-kb fragment of the rat skeletal muscle actin gene containing 1.2-kb of promoter, the first exon, first intron and 25-bp of the second exon was placed upstream to a....
8.	FAK (+/-)	The FAK (focal adhesion kinase) gene was mutated by inserting the neomycin phosphotransferase gene (neo (r)) in the beginning of its kinase domain. The targeted exon sp....
9.	Tg mdx (gene therap	A full-length dystrophin cDNA expression vector, pMDA, was used. The expression of the murine dystrophin cDNA was regulated by 6.5 kb of MCK (muscle creatine kinase) ge....
10.	trkC(TK) (+/-)	Genomic trkC sequences encoding the tyrosine kinase catalytic domain of gp145(trkC) (amino acid residues 573-630 were used to generate a targeting vector, pFRK100, by re....
11.	IGF-I (+/-)	A murine DBM genomic library, packaged in EMBL3 phage (Clontech), was screened using a random prime radiolabeled DNA probe specific to murine IGF-I (insulin-like growth....
12.	MyoD(m1)/MyoD(m1) (	To facilitate construction of an efficient replacement-type vector, the MyoD genomic locus was cloned from a lambda library constructed from DNA isolated from 129/Sv mic....
13.	fyn (-/-) & myelina	Fyn-kinase-deficient mice were generated by replacing the SH2, SH3 and tyrosine kinase regions of fyn by lacZ. The targeting vector, pHFZpr-NeoDT, (Yagi et al., 1993; G....
14.	c-mos (+/-)	Plasmid pNMV was constructing by inserting a neomycin-resistance gene (neo(r)) with a pgk-1 promoter in the same orientation as the c-mos gene into the BlnI (....
15.	ret-k (+/-)	A targeting construct was designed to insert a neo(R) gene into the c-ret gene, simultaneously deleting 0.8 kilobases (kb) of DNA, including the codon for an invariant l....
16.	MRF4 (-/-)	A genomic clone encompassing the MRF4 (Myf6, herculin) and Myf5 genes was isolated from a Balb/c mouse genomic library. The targeting vector was constructed by cloning....
17.	M-CK (-/-)