GENE KNOCKOUT DATABASE
Near complete inhibition of cerebrospinal fluid leukocyte influx in an acute cytokine-induced meningitis model
Tang, P. S. Frenette, R. O. Hynes, D. D. Wagner & T. N. Mayadas: Cytokine-induced Meningitis Is Dramatically Attenuated in Mice Deficient in Endothelial Selectins. J. Clin. Invest. 97, 2485-2490 (1996)
P- and E-selectin deficient mice
Extreme leukocytosis
Elevated cytokine levels
Increased bone marrow and spleen granulocytopoiesis
Erythropoiesis partially translocated to spleen
Virtual lack of leukocyte rolling
Low extravasation at sites of inflammation
High susceptability to opportunistic bacterial infection
| Line Name | DNA Construct | |
|---|---|---|
| 1. | P-selectin (+/-) | An amplified mouse genomic library constructed from the liver DNA of black agouti 129Sv mouse strain (kindly provided by Drs. H. Wu and R. Jaenisch (G00-100-762) was scr.... |
| 2. | P-selectin (-/-) | An amplified mouse genomic library constructed from the liver DNA of black agouti 129Sv mouse strain (kindly provided by Drs. H. Wu and R. Jaenisch (G00-100-762) was scr.... |
| 3. | Sele (+/-) | Murine E-selectin genomic clones were isolated from lambda libraries derived from either 129SV DNA (Stratagene, Incorporated) or BALB/c DNA (Clonetec, Incorporated). In.... |
| 4. | Sele (-/-) | Murine E-selectin genomic clones were isolated from lambda libraries derived from either 129SV DNA (Stratagene, Incorporated) or BALB/c DNA (Clonetec, Incorporated). In.... |
