Fyn
No overt phenotype
Normal paired pulse facilitation and post-tetanic potentiation
Markedly reduced calcium fluxes and abrogated proliferation of thymocytes in response to TCR-CD3 cross-linking
Reduced proliferation in thymoctes in response to Thy-1 cross-linking
Largely normal proliferation of mature splenic T cells in response to TCR-CD3 cross-linking
No reduction in proliferation induced by Thy-1 cross-linking in splenic T cells
Blunted long-term potentiation
Impaired spatial learning
Increased number of granule cells in the dentate gyrus
Increased number of pyramidal cells in the CA3 region
Impaired suckling behavior
Modified glomerular complex of the olfactory bulb
Undulated hippocampal cell-layer
Grant et al., 1992
Stein et al., 1992
Yagi et al., 1993
fyn, thymic isoform
Normal maturation sequence in peripheral T cells in response to stimulation of the TCR with mitogen or antigen Lack of response of thymocytes to stimulation of the TCR with mitogen or antigenFyn
Hypophosphorylation of nine proteins Reduced Focal adhesion kinase (FAK) activity FAK expressed in high levels in brain FAK not restricted to focal adhesion contacts FAK found in axons, dendrites, and intermediate filament cytoskeleton of astrocytes
Compensatory change in Src Family kinase activity found in brain extracts
-
Grant, 1995
-
Focal adhesion kinase in the brain: novel subcellular localization and specific regulation by Fyn tyrosine kinase in mutant mice.
Genes Dev 9, 1909-1921 (1995) [95377641]
(View Citation format,Abstract format,MEDLARS format,ASN.1 format,Save As..., or 30 Related MEDLINE citations)
| Line Name | DNA Construct | |
|---|---|---|
| 1. | Fyn (+/-) | Fyn-kinase-deficient mice were generated by replacing the SH2, SH3 and tyrosine kinase regions of fyn by lacZ. The targeting vector, pHFZpr-NeoDT, (G00-373-378) was ass.... |
| 2. | Fyn(z) (+/-) | In the fyn mutant mice the lacZ gene was inserted into exon 3 (fyn(z)) so that beta-galactosidase (beta-Gal) was expressed as a fusion protein with the Fyn unique region.... |
| 3. | fyn (LacZ/neo) (+/- | The LacZ gene was inserted into exon 3 in the in-frame so that it was expressed as the fusion protein with the Fyn-N-terminal region (a.a. 1-110); the region includes t.... |
| 4. | fyn (LacZ/neo (-/-) | The LacZ gene was inserted into exon 3 in the in-frame so that it was expressed as the fusion protein with the Fyn-N-terminal region (a.a. 1-110); the region includes t.... |
| 5. | Fyn(z) (-/-) | In the fyn mutant mice the lacZ gene was inserted into exon 3 (fyn(z)) so that beta-galactosidase (beta-Gal) was expressed as a fusion protein with the Fyn unique region.... |
| 6. | Fyn (-/-) | Fyn-kinase-deficient mice were generated by replacing the SH2, SH3 and tyrosine kinase regions of fyn by lacZ. The targeting vector, pHFZpr-NeoDT, (G00-373-378) was ass.... |
