GM-CSF
Normal development
No major abnormalities in hematopoiesis up to 12 weeks of age
Fertile
Development of abnormal lungs
Extensive lymphoid hyperplasia in airways
Progressive accumulation of surfactant and proteins in alveolar spaces
Extensive peribroncovascular infiltration of lymphocytes, predominantly B cells
Granular eosinophilic material and lamellar bodies in alveoli
Numerous large intraalveolar phagocytic macrophages
Subclinical lung infections involving bacterial or fungal organisms in some mice
Dranoff et al., 1994
Stanley et al., 1994
| Line Name | DNA Construct | |
|---|---|---|
| 1. | pPNT/GM-CSF(+/-) | A 3.2-kb Xba I fragment and 5.3-kb Hind III-Mbo I fragment derived from a 129S ES genomic library were inserted into the targeting vector pPNT. This construct was elect.... |
| 2. | GM-CSF (+/-) | The GM-CSF (granulocyte / macrophage colony-stimulating factor) targeting vector contained, from 5' to 3' in pIC20H, 900 bp of the GM-CSF gene promoter ( |
| 3. | pPNT/GM-CSF(-/-) | A 3.2-kb Xba I fragment and 5.3-kb Hind III-Mbo I fragment derived from a 129S ES genomic library were inserted into the targeting vector pPNT. This construct was elect.... |
| 4. | XEDA | The transgene used consists of a Moloney murine leukemia virus long terminal repeat (LTR) driving the gene for granulocyte/macrophage colony-stimulating factor (GM-CSF)..... |
| 5. | GM-CSF (-/-) | The GM-CSF (granulocyte / macrophage colony-stimulating factor) targeting vector contained, from 5' to 3' in pIC20H, 900 bp of the GM-CSF gene promoter ( |
