Neural-cell adhesion molecule (N-CAM)
Healthy
Fertile
Normal activity and motor abilities
10% reduction in overall brain weight
36% decline in size of olfactory bulb
Near total loss of protein-bound alpha-(2,8)-linked polysialic acid
Deficits in spatial learning
Low coat color chimeric mice produced, only wild-type transmitted
Severe growth retardation and morphological defects by E8.5-E9.5
Lethality
Similar lethal phenotype exhibited in embryos as heterozygous chimerasRabinowitz, 1996 Targeted mutation of Ncam to produce a secreted molecule results in a dominant embryonic lethality. Proc. Natl. Acad. Sci. U.S.A. 93, 6421-6424 (1996) [96270554]
| Line Name | DNA Construct | |
|---|---|---|
| 1. | N-CAM (+/-) | The repaired neo(r) gene of pMC1NeoployA (G00-354-270) was inserted, replacing most of exons 3 and 4 and the intron in between (detailed information on N-CAM-180 gene ta.... |
| 2. | N-CAM (-/-) | The repaired neo(r) gene of pMC1NeoployA (G00-354-270) was inserted, replacing most of exons 3 and 4 and the intron in between (detailed information on N-CAM-180 gene ta.... |
| 3. | N-CAM-180 (+/-) | A 129/Sv genomic library (Stratagene) was screened using a 860 bp |
| 4. | N-CAM-180 (-/-) | A 129/Sv genomic library (Stratagene) was screened using a 860 bp |
