GENE KNOCKOUT DATABASE
Normal ovulation efficiency in mice lacking tPA
Normal ovulation efficiency in mice lacking uPA
26% reduction of ovulation efficiency in mice lacking both tPA and uPA
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Leonardsson, 1995
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Ovulation efficiency is reduced in mice that lack plasminogen activator gene function: functional redundancy among physiological plasminogen activators.
Proc. Natl. Acad. Sci. U.S.A. 92, 12446-12450 (1995) [96109283]
Normal plasmin activity prior to ovulation in the ovaries of tissue-type PA (TPA) and PA inhibitor type I (PAI-1) knockout mice
Lower plasma concentrations of total cholesterol after 3 weeks of high fat cholesterol diet
Significant reduction in plasmin activity in the ovaries of urokinase-type PA (uPA) deficient mice prior to ovulation
Database link:
| Line Name | DNA Construct | |
|---|---|---|
| 1. | t-PA (-/-) | pNT.3't-PA was generated by cloning a 2.0-kb 3'-flanking |
| 2. | t-PA (+/-) | pNT.3't-PA was generated by cloning a 2.0-kb 3'-flanking |
| 3. | t-PA (-/-):u-PA (-/ | pNT.3't-PA was generated by cloning a 2.0-kb 3'-flanking |
| 4. | u-PA (+/-) | pNT.5'uk-PA was generated by cloning a 4.3-kb fragment from plasmid pBSMuPANeo[8.60riB]tk (provided by J. Degen; REG:742593), containing the 5'-flanking |
| 5. | tPA (-/-) follow-up | pNT.3't-PA was generated by cloning a 2.0-kb 3'-flanking |
| 6. | u-PA (-/-) | pNT.5'uk-PA was generated by cloning a 4.3-kb fragment from plasmid pBSMuPANeo[8.60riB]tk (provided by J. Degen; REG:742593), containing the 5'-flanking |
